PXD002966 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | OXIPEP: mass spectrometry strategy to characterize protein carbonylation. |
Description | Here we describe a peptide-centric approach (OXIPEP), where carbonylated residues within proteins are derivatized with biotin-hydrazide, enriched and identified by mass spectrometry. The strength of the method lies in an improved protocol for elution of biotinylated peptides from monomeric avidin resin using hot water (95C) and increased sensitivity achieved by reduction of sample losses during sample preparation and chromatographic steps. For the first time advanced bioinformatics analysis utilising diagnostic biotin fragment ions is used to facilitate semi-automatic in silico evaluation of biotin labelling and to increase the reliability of carbonyl peptide identification. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-18 |
AnnouncementXML | Submission_2018-10-18_11:29:09.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adelina Rogowska-Wrzesinska |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-24 03:58:15 | ID requested | |
⏵ 1 | 2018-10-18 11:29:10 | announced | |
2 | 2024-10-22 04:26:00 | announced | 2024-10-22: Updated project metadata. |
Publication List
Havelund JF, Wojdyla K, Davies MJ, Jensen ON, M, ΓΈ, ller IM, Rogowska-Wrzesinska A, A biotin enrichment strategy identifies novel carbonylated amino acids in proteins from human plasma. J Proteomics, 156():40-51(2017) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: protein carbonylation, biotin-hydrazide, bovine serum albumine, human plasma |
Contact List
Ole N. Jensen |
contact affiliation | Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark |
contact email | jenseno@bmb.sdu.dk |
lab head | |
Adelina Rogowska-Wrzesinska |
contact affiliation | University of Southern Denmark |
contact email | adelinar@bmb.sdu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002966
- Label: PRIDE project
- Name: OXIPEP: mass spectrometry strategy to characterize protein carbonylation.