PXD002965-2

PXD002965 is an original dataset announced via ProteomeXchange.

Title	RACK1 interactome in drosophila S2 cells (FLAG-tag), using DDA and DIA (SWATH) approaches
Description	Receptor for Activated C kinase 1 (RACK1) is a scaffold protein that has been found in association with several signaling complexes, and with the 40S subunit of the ribosome. Data presented here allowed a proteomic characterization of the interactome of RACK1 in drosophila S2 cells. We carried out Label-Free quantitation using both Data-Dependent (DDA) and Data-Independent Acquisition (DIA, SWATH). This study is a 2-factor experiment, where control samples (RACK1 overexpressed with a HA tag) were compared to IP samples (RACK1 overexpressed with a FLAG tag), in either mock or infected cells. Each of these 6 conditions were performed in biological triplicates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:26:25.921.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-24 00:43:28	ID requested
1	2017-05-29 05:29:57	announced
⏵ 2	2024-10-22 04:26:28	announced	2024-10-22: Updated project metadata.

Kuhn L, Majzoub K, Einhorn E, Chicher J, Pompon J, Imler JL, Hammann P, Meignin C, Using SWATH-MS. G3 (Bethesda), 7(7):2249-2258(2017) [pubmed]

10.1534/g3.117.042564;

curator keyword: Biological

submitter keyword: Affinity purification, RNA interference,RACK1, interactomics, Drosophila

Philippe Hammann
contact affiliation	CNRS, IBMC, FRC1589, Proteomics Core Facility
contact email	p.hammann@ibmc-cnrs.unistra.fr
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD002965

PRIDE project URI

• PRIDE
  1. PXD002965
     1. Label: PRIDE project
     2. Name: RACK1 interactome in drosophila S2 cells (FLAG-tag), using DDA and DIA (SWATH) approaches