PXD002933 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The E. coli type III secreted effectors NleB and EspL interact with the microtubule associated protein ensconsin to disrupt vesicle trafficking, part 1 |
Description | Enteropathogenic and enterohaemorrhagic E. coli (EPEC and EHEC) translocate a set of type III effector proteins into host cells that are critical for bacterial virulence. These effectors subvert normal host pathways by interacting with a variety of targets within the cell, but the binding partners and mechanism of action of the majority of effectors are not understood. We identified the microtubule associated protein, ensconsin, as a novel target for two EPEC/EHEC effectors. We found that the secreted effectors NleB and EspL bind host ensconsin and work synergistically to paralyze kinesin-based intracellular vesicular transport. Our findings demonstrate that EPEC/EHEC encode multiple effectors that control intracellular vesicle movement and suggest a simple strategy for broad-based immobilization of host cells by pathogens. |
HostingRepository | PRIDE |
AnnounceDate | 2016-12-22 |
AnnouncementXML | Submission_2016-12-21_23:47:53.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Leonard Foster |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ FT; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-17 00:57:07 | ID requested | |
⏵ 1 | 2016-12-21 23:47:54 | announced | |
2 | 2017-10-24 03:12:08 | announced | Updated project metadata. |
Publication List
Law RJ, Law HT, Scurll JM, Scholz R, Santos AS, Shames SR, Deng W, Croxen MA, Li Y, de Hoog CL, van der Heijden J, Foster LJ, Guttman JA, Finlay BB, Quantitative Mass Spectrometry Identifies Novel Host Binding Partners for Pathogenic Escherichia coli Type III Secretion System Effectors. J Proteome Res, 15(5):1613-22(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Human, E. coli, host-pathogen, effector |
Contact List
Leonard James Foster |
contact affiliation | Centre for High-Throughput Biology, University of British Columbia |
contact email | foster@chibi.ubc.ca |
lab head | |
Leonard Foster |
contact affiliation | Centre for High Throughput Biology |
contact email | ljfoster@interchange.ubc.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002933
- Label: PRIDE project
- Name: The E. coli type III secreted effectors NleB and EspL interact with the microtubule associated protein ensconsin to disrupt vesicle trafficking, part 1