PXD002920-2

PXD002920 is an original dataset announced via ProteomeXchange.

Title	Early events on compatible and incompatible grapevine interaction with Plasmopara viticola: a proteomic perspective of cultivar specific behavior
Description	Grapevine downy mildew is an important disease affecting crop production and causing severe losses. To identify genotype-dependent responses towards this pathogen and to explore the molecular mechanisms involved in grapevine-P. viticola resistance, we have conducted a proteomic analysis of leaf samples from resistant and susceptible grapevine genotypes prior and post-inoculation with the pathogen. Proteins were analyzed by quantitative two-dimensional differential gel electrophoresis (2D-DIGE). The analysis able to identified 50 unique proteins. Functional analysis showed that photosynthesis and metabolism were the main categories differentiating genotypes at 0h and that P. viticola-responsive proteins were mainly involved in photosynthesis, carbohydrate metabolism, stress and defense responses and redox homeostasis. ROS production, total antioxidant capacity and lipid peroxidation on both genotypes were determined and together with the proteome data suggest that Regent presents a strict balance between ROS control and signaling leading to plant cell death activation. Our data reveals the genotype-dependent modulation of plant metabolism and defense responses providing new insights into underlying molecular processes of grapevine resistance against the downy mildew fungus.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:25:27.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joana Martins
SpeciesList	scientific name: Vitis vinifera (Grape); NCBI TaxID: 29760;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-15 03:51:51	ID requested
1	2017-01-04 03:55:32	announced
⏵ 2	2024-10-22 04:25:27	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Grapevine

downy mildew

Resistance

2D-DIGE

plant cell death

Ana Varela Coelho
contact affiliation	Instituto de Tecnologia Química e Biológica. Lab of Proteomic of Non-model Organism
contact email	varela@itqb.unl.pt
lab head
Joana Martins
contact affiliation	ITQB-UNL
contact email	joanam@itqb.unl.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD002920

PRIDE project URI

• PRIDE
  1. PXD002920
     1. Label: PRIDE project
     2. Name: Early events on compatible and incompatible grapevine interaction with Plasmopara viticola: a proteomic perspective of cultivar specific behavior