PXD002854 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Plasma proteome profiling to assess human health and disease |
Description | Proteins in the circulatory system mirror an individual´s physiology. They are easy to obtain and consequently, plasma and serum are the predominant matrices for diagnostic analyses in daily clinical practice. Protein levels are generally determined using single protein immuno-assays. Mass spectrometry (MS)-based proteomics of this body fluid is challenging due to the high dynamic range of protein abundances. Here we introduce a rapid and robust single-run proteomic workflow that enables the quantitative analysis of hundreds of plasma proteomes from single finger pricks with 20 min gradients. The apolipoprotein family, inflammatory markers such as C-reactive protein, gender-related proteins and more than 40 FDA approved biomarkers are reproducibly quantified (CV<20% with label-free quantification). Furthermore, we functionally interpret a 1000 protein, quantitative plasma proteome obtained by simple peptide pre-fractionation. ‘Plasma proteome profiling’ delivers an informative portrait of a person’s health state and we envision its large-scale use in biomedicine. |
HostingRepository | PRIDE |
AnnounceDate | 2016-02-22 |
AnnouncementXML | Submission_2016-02-22_05:43:05.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mario Oroshi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-06 23:49:22 | ID requested | |
⏵ 1 | 2016-02-22 05:43:06 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: Plasma proteome profile, mass spectrometry, clinic, disease, human, apolipoproteins, blood analysis |
Contact List
Matthias Mann |
contact affiliation | Proteomics and Signal Transduction Max Planck Institute of Biochemistry Am Klopferspitz 18 D-82152 Martinsried |
contact email | mmann@biochem.mpg.de |
lab head | |
Mario Oroshi |
contact affiliation | Proteomics |
contact email | oroshi@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002854 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002854
- Label: PRIDE project
- Name: Plasma proteome profiling to assess human health and disease