PXD002825-2

PXD002825 is an original dataset announced via ProteomeXchange.

Title	Down-regulation of Vimentin Increased Drug Resistance in Ovarian Cancer Cells
Description	Cisplatin and other platinum-based drugs are widely used in the treatment of ovarian cancer, but most patients acquire the drug resistance that greatly compromises the efficacy of drugs. Understanding the mechanism of drug resistance is important for finding new therapeutic approaches. In the present study, we found that the expression of vimentin was down-regulated in drug-resistance ovarian cancer A2780-DR and SKOV-3/DDP cells compared to the drug sensitive A2780 and SKOV-3 cells. Overexpression of vimentin in A2780-DR cells markedly increased their sensitivity to cisplatin, whereas knockdown of vimentin in A2780 cells increased the resistance to cisplatin, demonstrating that vimentin plays an important role in cisplatin resistance. Quantitative proteomic analysis identified 95 differentially expressed proteins between A2780-VIM-KN and control cells, which involved in many cellular processes. Down-regulation of endocytic proteins and the up-regulation of exocytic proteins were proposed to contribute the decreased cisplatin accumulation in A2780-VIM-KN cells. Cancer stem cell markers were found to be up-regulated in A2780-VIM-KN cells which were more facile to form spheroids as compared to control cells. Our results also showed that down-regulation of vimentin increased the 14-3-3 mediated retention of Cdc25C in the cytoplasm, leading to inactivation of Cdk1 and the prolonged G2 arrest that allows the longer period of time for cells to repair cisplatin-damaged DNA. Taken together, the down-regulation of vimentin enhances cells’ resistance to cisplatin via mediating multiple cellular processes, suggesting that vimentin is a potential target for treatment of drug resistant ovarian cancer.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:25:39.043.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huo Yi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-01 00:26:37	ID requested
1	2018-10-24 10:56:14	announced
⏵ 2	2024-10-22 04:25:47	announced	2024-10-22: Updated project metadata.

Huo Y, Zong Z, Wang Q, Zhang Z, Deng H, ISG15 silencing increases cisplatin resistance via activating p53-mediated cell DNA repair. Oncotarget, 8(64):107452-107461(2017) [pubmed]

10.18632/oncotarget.22488;

curator keyword: Biomedical

submitter keyword: Ovarian cancer cell line

Drug-resistance

Proteomics

Vimentin

Cisplatin

Haiteng Deng
contact affiliation	MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China
contact email	dht@biomed.tsinghua.edu.cn
lab head
Huo Yi
contact affiliation	Tsinghua University
contact email	cookiesyiyi@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002825
     1. Label: PRIDE project
     2. Name: Down-regulation of Vimentin Increased Drug Resistance in Ovarian Cancer Cells