PXD002816-1

PXD002816 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Differential stoichiometry among core ribosomal proteins
Description	Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its dysregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testing such variability requires direct and precise quantification of RPs. We used mass-spectrometry to directly quantify RPs across monosomes and polysomes of mouse embryonic stem cells (ESC) and budding yeast. Our data show that the stoichiometry among core RPs in wild-type yeast cells and ESC depends both on the growth conditions and on the number of ribosomes bound per mRNA. Furthermore, we find that the fitness of cells with a deleted RP-gene is inversely proportional to the enrichment of the corresponding RP in polysomes. Together, our findings support the existence of ribosomes with distinct protein composition and physiological function.
HostingRepository	MassIVE
AnnounceDate	2017-01-23
AnnouncementXML	Submission_2017-01-23_07:47:57.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikolai Slavov
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; scientific name: Saccharomycetales; common name: budding yeasts; NCBI TaxID: 4892;
ModificationList	Phospho
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-28 12:28:06	ID requested
⏵ 1	2017-01-23 07:47:58	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: ribosomes

submitter keyword: ribosomes

Contact List

principal_investigator
lab head
Nikolai Slavov
contact affiliation	Harvard University
contact email	nslavov@alum.mit.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079280