PXD002678-1

PXD002678 is an original dataset announced via ProteomeXchange.

Title	Gluten-specific antibodies of celiac disease gut plasma cells recognize long proteolytic fragments that typically harbor T-cell epitopes
Description	This study aimed to identify proteolytic fragments of gluten proteins recognized by recombinant IgG1 monoclonal antibodies generated from single IgA plasma cells of celiac disease lesions. Peptides bound by monoclonal antibodies in complex gut-enzyme digests of gluten treated with the deamidating enzyme transglutaminase 2, were identified by mass spectrometry after antibody pull-down with protein G beads. The antibody bound peptides were long deamidated peptide fragments that contained the substrate recognition sequence of transglutaminase 2. Characteristically, the fragments contained epitopes with the sequence QPEQPFP and variants thereof in multiple copies, and they typically also harbored many different gluten T-cell epitopes. In the pull-down setting where antibodies were immobilized on a solid phase, peptide fragments with multivalent display of epitopes were targeted. This scenario resembles the situation of the B-cell receptor on the surface of B cells. Conceivably, B cells of celiac disease patients select gluten epitopes that are repeated multiple times in long peptide fragments generated by gut digestive enzymes. As the fragments also contain many different T-cell epitopes, this will lead to generation of strong antibody responses by effective presentation of several distinct T-cell epitopes and establishment of T-cell help to B cells.
HostingRepository	PRIDE
AnnounceDate	2016-05-10
AnnouncementXML	Submission_2016-05-10_04:42:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Siri Dørum
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-06 02:40:09	ID requested
⏵ 1	2016-05-10 04:42:28	announced
2	2024-10-22 03:59:17	announced	2024-10-22: Updated project metadata.

D, ø, rum S, Steinsb, ø Ø, Bergseng E, Arntzen M Ø, de Souza GA, Sollid LM, Gluten-specific antibodies of celiac disease gut plasma cells recognize long proteolytic fragments that typically harbor T-cell epitopes. Sci Rep, 6():25565(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Celiac disease, gliadin, monoclonal antibodies, epitopes, LC-MSMS

Gustavo A. de Souza
contact affiliation	1. Centre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital - Rikshospitalet, 0424 Oslo, Norway 2. Proteomics Core Facility, Oslo University Hospital-Rikshospitalet, 0424 Oslo, Norway
contact email	g.a.d.souza@medisin.uio.no
lab head
Siri Dørum
contact affiliation	Centre for Immune Regulation, University of Oslo
contact email	siri.dorum@rr-research.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002678
     1. Label: PRIDE project
     2. Name: Gluten-specific antibodies of celiac disease gut plasma cells recognize long proteolytic fragments that typically harbor T-cell epitopes