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PXD002535-1

PXD002535 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMiddle-down MS analysis of intact and clipped histone H3 proteoforms
DescriptionThere is growing evidence that proteolytic cleavage of histone N-terminal tails, known as histone clipping, influences nucleosome dynamics and functional properties. Using middle-down MS analysis we characterized intact and proteolytically processed histone H3 proteoforms isolated from human hepatocarcinoma cell line HepG2/C3A grown in 3D culture.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:28:13.398.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndrey Tvardovskiy
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; phosphorylated residue; acetylated residue; dimethylated residue; N6,N6,N6-trimethyl-L-lysine
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-14 01:15:48ID requested
12024-10-07 13:28:14announced
Publication List
Tvardovskiy A, Wrzesinski K, Sidoli S, Fey SJ, Rogowska-Wrzesinska A, Jensen ON, Top-down and Middle-down Protein Analysis Reveals that Intact and Clipped Human Histones Differ in Post-translational Modification Patterns. Mol Cell Proteomics, 14(12):3142-53(2015) [pubmed]
10.1074/mcp.m115.048975;
Keyword List
curator keyword: Biological
submitter keyword: liver, Hepg2/C3A,Middle-down MS, Histone post-translational modifications
Contact List
Ole Nørregaard Jensen
contact affiliationDepartment of Biochemistry and Molecular Biology, Protein Research Group, University of Southern Denmark
contact emailjenseno@bmb.sdu.dk
lab head
Andrey Tvardovskiy
contact affiliationUniversity of Southern Denmark
contact emailantvar@bmb.sdu.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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