PXD002500-3

PXD002500 is an original dataset announced via ProteomeXchange.

Title	The spider clot proteome - The spider hemolymph clot proteome reveals high concentrations of hemocyanin and von Willebrand factor-like proteins
Description	Arthropods include chelicerates, crustaceans, and insects that all have open circulation systems and thus require different properties of their coagulation system than vertebrates. Although the clotting reaction in the chelicerate horseshoe crab has been described in details, the overall protein composition of the resulting clot has not been analysed for any of the chelicerates. The largest class among the chelicerates is the arachnids, which includes spiders, ticks, mites, and scorpions. Here, we use a mass spectrometry-based approach to characterize the spider hemolymph clot proteome from the Brazilian whiteknee tarantula, Acanthoscurria geniculate. We focused on the insoluble part of the clot and demonstrated high concentrations of proteins homologous to the hemostasis-related and multimerization-prone von Willebrand factor. These proteins, which include hemolectins and vitellogenin homologous, were previously identified as essential components of the hemolymph clot in crustaceans and insects. Their presence in the spider hemolymph clot suggests that the origin of these proteins’ function in coagulation predates the split between chelicerates and mandibulata. The clot proteome reveals that the major proteinaceous component is the oxygen-transporting and phenoloxidase-displaying abundant hemolymph protein hemocyanin, suggesting that this protein also plays a role in clot biology. In general, many of the identified clot-proteins are related to the innate immune system, and our results support the previously suggested crosstalk between immunity and coagulation in arthropods.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:08:28.273.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristian Wejse Sanggaard
SpeciesList	scientific name: Acanthoscurria geniculata; NCBI TaxID: 575412;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-08 01:55:22	ID requested
1	2017-01-11 04:36:21	announced
2	2017-10-24 06:14:49	announced	Updated project metadata.
⏵ 3	2024-10-22 04:08:29	announced	2024-10-22: Updated project metadata.

10.1016/j.bbapap.2015.11.004;

Sanggaard KW, Dyrlund TF, Bechsgaard JS, Scavenius C, Wang T, Bilde T, Enghild JJ, The spider hemolymph clot proteome reveals high concentrations of hemocyanin and von Willebrand factor-like proteins. Biochim Biophys Acta, 1864(2):233-41(2016) [pubmed]

curator keyword: Biological

submitter keyword: innate immunity,LC-MS/MS, proteomics, chelicerates, spiders, coagulation, transcriptomics, arthropods

Jan J. Enghild
contact affiliation	Interdisciplinary Nanoscience Center and Department of Molecular Biology and Genetics, Aarhus University, Denmark
contact email	jje@mbg.au.dk
lab head
Kristian Wejse Sanggaard
contact affiliation	Department of Molecular Biology and Genetics
contact email	kws@mbg.au.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002500
     1. Label: PRIDE project
     2. Name: The spider clot proteome - The spider hemolymph clot proteome reveals high concentrations of hemocyanin and von Willebrand factor-like proteins