PXD002480 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Facile Discovery of Cell-surface Protein Targets of Cancer Cell Aptamers |
Description | Cancer biomarker discovery constitutes a frontier in cancer research. In recent years, cell-binding aptamers have become useful molecular probes for biomarker discovery. However, there are few successful examples, and the critical barrier resides in the identification of the cell-surface protein targets for the aptamers, where only a limited number of aptamer targets have been identified so far. Herein, we developed a universal SILAC-based quantitative proteomic method for target discovery of cell-binding aptamers. The method allowed for distinguishing specific aptamer-binding proteins from non-specific proteins based on abundance ratios of proteins bound to aptamer-carrying bait and control bait. In addition, we employed fluorescently labeled aptamers for monitoring and optimizing the binding conditions. We were able to identify and validate selectin L and integrin 4 as the protein targets for two previously reported aptamers, Sgc-3b and Sgc-4e, respectively. This strategy should be generally applicable for the discovery of protein targets for other cell-binding aptamers, which will promote the applications of these aptamers. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-28 |
AnnouncementXML | Submission_2022-02-28_02:21:41.126.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tao Bing |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-07-06 01:33:38 | ID requested | |
⏵ 1 | 2022-02-28 02:21:41 | announced | |
Publication List
Bing T, Shangguan D, Wang Y, Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers. Mol Cell Proteomics, 14(10):2692-700(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Affinity proteomics, Aptamers, Cancer biomarker(s), Quantification, SILAC. |
Contact List
Yinsheng Wang |
contact affiliation | Department of Chemistry, University of California, Riverside, CA92521-0403, USA. |
contact email | Yinsheng.Wang@ucr.edu |
lab head | |
Tao Bing |
contact affiliation | Institute of Chemistry, Chinese Academy of Sciences |
contact email | bingtao@iccas.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD002480 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002480
- Label: PRIDE project
- Name: Facile Discovery of Cell-surface Protein Targets of Cancer Cell Aptamers