PXD002424-1

PXD002424 is an original dataset announced via ProteomeXchange.

Title	Human cerebrospinal fluid exosome, LC-MSMS
Description	Quantitative proteomics analysis of exosomes isolated from cerebrospinal fluid of neuromyelitis optica patients detected potential distinctive disease markers capable of differentiating from multiple sclerosis and idiopathic longitudinally extensive transverse myelitis. Exosomes were enriched with high purity using differential centrifugation from pooled cerebrospinal fluids. The sample pooling strategy included pre-classification by cytokine level, which improved target molecule identification by providing enhanced fold change ratios and lower p-values when the subsets with high-cytokines were compared to each other. In addition to the spectral counts and label-free quantification, orthogonal quantitative validation was performed using tandem mass spectrometry analysis via UPLC and Orbitrap or Q-Exactive Hybrid Quadrupole-Orbitrap instrumentation. The proteomic datasets with 442 significant exosomal proteins detected putative disease specific biomarkers, glial fibrillary acidic protein and fibronectin for neuromyelitis optica and multiple sclerosis, respectively. Additional investigation of expressions of glial fibrillary acidic protein and fibronectin revealed their presence in intact exosomes as detected by flow cytometry. ELISA measurement of glial fibrillary acidic protein within individual samples demonstrated diagnostic applicability by appropriately clustering disease groups. Expression levels of target molecules from label-free quantification were in good agreement with those from Western blotting. Finally, MetaCore pathway analysis of identified proteins supported the involvement of these proteins in disease progression via neurological pathway involvement. This comprehensive study suggests that the exosomal proteomics approach of cerebrospinal fluid can be applied to the identification and characterization of inflammatory disorders in central nervous system.
HostingRepository	PRIDE
AnnounceDate	2016-09-27
AnnouncementXML	Submission_2016-09-26_21:12:27.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002424
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jingyun Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-25 01:07:56	ID requested
⏵ 1	2016-09-26 21:12:29	announced

Lee J, McKinney KQ, Pavlopoulos AJ, Han MH, Kim SH, Kim HJ, Hwang S, Exosomal proteome analysis of cerebrospinal fluid detects biosignatures of neuromyelitis optica and multiple sclerosis. Clin Chim Acta, 462():118-126(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Cerebrospinal fluid, exosome, neuromyelitis optica, multiple sclerosis

Sunil Hwang
contact affiliation	Cannon Research Center, Mass Spectrometry Core Facility, Carolinas HealthCare System, United States.
contact email	sunil.hwang@carolinas.org
lab head
Jingyun Lee
contact affiliation	Carolinas HealthCare System
contact email	jingyun.lee1973@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002424
     1. Label: PRIDE project
     2. Name: Human cerebrospinal fluid exosome, LC-MSMS