PXD002399-1

PXD002399 is an original dataset announced via ProteomeXchange.

Title	LMTK3 Represses Tumour Suppressor-Like Genes through Chromatin Remodeling in Breast Cancer
Description	LMTK3 is an oncogenic receptor tyrosine kinase (RTK) implicated in various types of cancer including breast, lung, gastric and colorectal. It is localized in different cellular compartments but its nuclear function has not been investigated thus far. We have mapped LMTK3 binding across the genome using ChIP-seq and found that LMTK3 binding events are correlated with repressive chromatin markers. We further identified KRAB associated protein-1 (KAP1) as a binding partner of LMTK3. The LMTK3/KAP1 interaction is stabilized by PP1α, which suppresses KAP1 phosphorylation specifically at LMTK3-associated chromatin regions, inducing chromatin condensation and resulting in transcriptional repression of LMTK3-bound tumour suppressor-like genes. Furthermore, LMTK3 functions at enhancer regions in tethering the chromatin to the nuclear periphery, resulting in H3K9me3 modification and gene silencing. In summary, we propose a model where a scaffolding function of nuclear LMTK3 promotes cancer progression through chromatin remodeling, revealing a previously undescribed mechanism of RTK activity.
HostingRepository	PRIDE
AnnounceDate	2019-10-30
AnnouncementXML	Submission_2019-10-30_08:57:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georgios Giamas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-19 03:39:20	ID requested
⏵ 1	2019-10-30 08:57:30	announced
2	2024-10-22 04:11:48	announced	2024-10-22: Updated project metadata.

Xu Y, Zhang H, Nguyen VT, Angelopoulos N, Nunes J, Reid A, Buluwela L, Magnani L, Stebbing J, Giamas G, LMTK3 Represses Tumor Suppressor-like Genes through Chromatin Remodeling in Breast Cancer. Cell Rep, 12(5):837-49(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: LMTK3 Chromatin Breast Cancer

Georgios Giamas
contact affiliation	Imperial College London
contact email	g.giamas@imperial.ac.uk
lab head
Georgios Giamas
contact affiliation	Imperial College
contact email	g.giamas@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD002399

PRIDE project URI

• PRIDE
  1. PXD002399
     1. Label: PRIDE project
     2. Name: LMTK3 Represses Tumour Suppressor-Like Genes through Chromatin Remodeling in Breast Cancer