PXD002346-1

PXD002346 is an original dataset announced via ProteomeXchange.

Title	Urinary polypeptide ETD/CID analysis, part 2
Description	Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and protein degradation products, which have been filtered from blood plasma, thus urine has strong potential as a source for novel clinical diagnostics in disease. In this study, we examine the urinary peptidome from normal healthy women during pregnancy, to demonstrate that peptides are readily observed. We utilise the dissociation method, electron transfer dissociation (ETD) to increase the identification rate of the peptides present within these samples, as the polypeptide species observed in these samples are large and highly charged. An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via the use of ETD.
HostingRepository	PRIDE
AnnounceDate	2016-02-15
AnnouncementXML	Submission_2016-02-15_05:53:07.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002346
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sarah Hart
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-10 05:33:01	ID requested
⏵ 1	2016-02-15 05:53:08	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Peptidome, urine, electron transfer dissociation, pregnancy, non-tryptic peptides

Sarah Hart
contact affiliation	Keele University
contact email	s.r.hart@keele.ac.uk
lab head
Sarah Hart
contact affiliation	Keele University
contact email	s.r.hart@keele.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002346

PRIDE project URI

• PRIDE
  1. PXD002346
     1. Label: PRIDE project
     2. Name: Urinary polypeptide ETD/CID analysis, part 2