PXD002135-2

PXD002135 is an original dataset announced via ProteomeXchange.

Title	ATR inhibition rewires cellular signaling networks in response to pathologic replication stress
Description	DNA replication stress is the major cause of genomic instability in human cells. The ataxia telangiectasia and Rad3-related kinase (ATR) plays an essential role in the cellular response to replication stress and inhibition of ATR has emerged as therapeutic strategy for the treatment of cancer. However, the ATR-dependent signaling in the cellular response to replication stress has not been systematically investigated. Here, we employ quantitative mass spectrometry-based proteomics to decipher the ATR-dependent phosphorylation events in response to pathological replication stress induced by hydroxyurea. Our data define the substrate spectrum of ATR and identify several novel putative ATR substrates. In addition, we demonstrate that ATR-inhibition fundamentally rewires cellular signaling networks leading to the prominent activation of different pathways including the ATM-driven double strand break repair.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:23:47.279.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Petra Beli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-01 08:26:36	ID requested
1	2015-11-24 00:16:25	announced
⏵ 2	2024-10-22 04:23:48	announced	2024-10-22: Updated project metadata.

Wagner SA, Oehler H, Voigt A, Dalic D, Freiwald A, Serve H, Beli P, ATR inhibition rewires cellular signaling networks induced by replication stress. Proteomics, 16(3):402-16(2016) [pubmed]

10.1002/pmic.201500172;

curator keyword: Biomedical

submitter keyword: ATR, Phosphoproteomics,Phosphorytion

Petra Beli
contact affiliation	Institute of Molecular Biology (IMB), Mainz, Germany
contact email	p.beli@imb-mainz.de
lab head
Petra Beli
contact affiliation	Institute of Molecular Biology (IMB), Mainz, Germany
contact email	p.beli@imb-mainz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002135
     1. Label: PRIDE project
     2. Name: ATR inhibition rewires cellular signaling networks in response to pathologic replication stress