PXD002112-2

PXD002112 is an original dataset announced via ProteomeXchange.

Title	Feedback circuitry between miR-218 repression and RTK activation in glioblastoma
Description	Receptor tyrosine kinase pathway signalings plays a central role in the growth and progression of glioblastoma, a highly aggressive group of brain tumors. We recently reported that miR-218 repression, an essentially uniform feature of human GBM, directly promotes RTK hyperactivation by increasing the expression of key positive signaling effectors, including EGFR, PLCr1, PIK3CA and ARAF. However, enhanced RTK signaling is known to activate compensatory inhibitory feedback mechanisms in both normal and cancer cells. We demonstrate here that miR-218 repression in GBM cells also increases the abundance of additional up stream and downstream signaling mediators, including PDGFRa, RSK2, and S6K1, which collectively funciton to alleviate inhibitory RTK feedback regulation. In turn, RTK signaling suppresses miR-218 expression via STAT3, which binds to the miR-218 locus, along with BCLAF1, to repress its expression. These data identify novel interacting feedback loops by which miR-218 repression promotes increased RTK signaling in high-grade gliomas.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:24.424.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yangpeiwei Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-28 01:44:07	ID requested
1	2022-03-01 06:56:33	announced
⏵ 2	2024-10-22 05:34:24	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: RTK, Glioblastoma, STAT3,miR-218

Marie Celeste Simon
contact affiliation	Abramson Family Cancer Research Institute, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, the United States
contact email	celeste2@mail.med.upenn.edu
lab head
Yangpeiwei Huang
contact affiliation	University of Pennsylvania
contact email	huangypw@mail.med.upenn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD002112

PRIDE project URI

• PRIDE
  1. PXD002112
     1. Label: PRIDE project
     2. Name: Feedback circuitry between miR-218 repression and RTK activation in glioblastoma