PXD002096-2

PXD002096 is an original dataset announced via ProteomeXchange.

Title	The influence of simulated microgravity on the proteome of Daphnia magna
Description	Background/Objectives: The waterflea Daphnia is an interesting candidate for biore- generative life support systems (BLSS). These animals are particularly promising be- cause of their central role in the limnic food web and its mode of reproduction. How- ever, the response of Daphnia to altered gravity conditions has to be investigated, especially on the molecular level, to evaluate the suitability of Daphnia for BLSS in space. Methods: In this study, we applied a proteomic approach to identify key proteins and pathways involved in the response of Daphnia to simulated microgravity gener- ated by a 2D-clinostat. We analysed 5 biological replicates using 2D-DIGE proteomic analysis. Results: We identified 109 protein spots differing in intensity (p < 0.05). Substan- tial fractions of these proteins are involved in actin microfilament organisation, in- dicating the disruption of cytoskeletal structures during clinorotation. Furthermore, proteins involved in protein folding were identified, suggesting altered gravity in- duced break-down of protein structures in general. In addition, simulated micro- gravity increased the abundance of energy metabolism related proteins, indicating an enhanced energy demand of Daphnia. Conclusion: The affected biological processes were also described in other studies using different organisms and systems either aiming to simulate microgravity con- ditions or providing real microgravity conditions. Moreover, most of the Daphnia protein sequences are well conserved throughout taxa, indicating that the response to altered gravity conditions in Daphnia follows a general concept.
HostingRepository	PRIDE
AnnounceDate	2015-09-08
AnnouncementXML	Submission_2016-06-01_03:54:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathrin Otte
SpeciesList	scientific name: Daphnia magna; NCBI TaxID: 35525;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-22 00:54:51	ID requested
1	2015-09-09 05:16:07	announced
⏵ 2	2016-06-01 03:54:17	announced	Updated publication reference for DOI(s): http://dx.doi.org/10.1038/npjmgrav.2015.16.
3	2017-08-06 23:56:42	announced	Updated project metadata.
4	2023-11-14 08:48:50	announced	2023-11-14: Updated project metadata.

http://dx.doi.org/10.1038/npjmgrav.2015.16;

curator keyword: Biological

submitter keyword: Daphnia, Proteomics, clinorotation, bioregenerative life support system, functional weightlessness

Georg Arnold
contact affiliation	Gene Centre, LMU Munich
contact email	arnold@genzentrum.lmu.de
lab head
Kathrin Otte
contact affiliation	A
contact email	otte@bio.lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002096
     1. Label: PRIDE project
     2. Name: The influence of simulated microgravity on the proteome of Daphnia magna