PXD002090-1

PXD002090 is an original dataset announced via ProteomeXchange.

Title	Development of a Mass Spectrometry-Based Quantitation Assay for Human Body Fluid Proteins using a Wheat Germ Cell-Free Synthesis System
Description	In this study, we have developed a large-scale SRM assay targeting human body fluid proteins based on publicly available proteomics data. We first aimed to produce, in a high throughput manner, a peptide reference library. For this purpose, we selected 2,201 targeted peptides representing 1,215 proteins from previous published studies, and in vitro synthesized them as peptide concatamers (QconCATs) using a wheat germ cell-free system.
HostingRepository	PRIDE
AnnounceDate	2016-05-24
AnnouncementXML	Submission_2016-05-24_04:07:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nobuaki Takemori
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine
Instrument	Q TRAP

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-20 06:01:33	ID requested
⏵ 1	2016-05-24 04:07:52	announced

Takemori N, Takemori A, Tanaka Y, Ishizaki J, Hasegawa H, Shiraishi A, Ohashi Y, High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system. Mol Biosyst, 12(8):2389-93(2016) [pubmed]

submitter keyword: Human body fluid proteins, Reference peptide library, Targeted proteomics, Wheat germ cell-free system

Nobuaki Takemori
contact affiliation	Ehime University, JAPAN
contact email	takemori@m.ehime-u.ac.jp
lab head
Nobuaki Takemori
contact affiliation	Ehime University, JAPAN
contact email	takemori@m.ehime-u.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD002090

PRIDE project URI

• PRIDE
  1. PXD002090
     1. Label: PRIDE project
     2. Name: Development of a Mass Spectrometry-Based Quantitation Assay for Human Body Fluid Proteins using a Wheat Germ Cell-Free Synthesis System