PXD002079 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic analysis of cell-based resistance to BRAF inhibitor therapy in melanoma |
Description | The treatment of melanoma by targeted inhibition of the mutated kinase BRAF with small molecules only temporarily suppresses metastatic disease. In the face of chemical inhibition tumor plasticity, both innate and adaptive, promotes survival through the biochemical and genetic reconfiguration of cellular pathways that can engage proliferative and migratory systems. To investigate this process high-resolution mass spectrometry was used to characterize the phosphoproteome of this transition in vitro. A simple and accurate, label-free quantitative method was used to localize and quantitate thousands of phosphorylation events. We also correlated changes in the phosphoproteome with the proteome to more accurately determine changes in the activity of regulatory kinases determined by kinase landscape profiling. The abundance of phosphopeptides with sites that function in cytoskeletal regulation, GTP/GDP exchange, Protein Kinase C, IGF signaling and melanosome maturation were highly divergent after transition to a drug resistant phenotype. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-24 |
AnnouncementXML | Submission_2018-10-24_09:06:47.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Robert Parker |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-04-20 01:26:44 | ID requested | |
⏵ 1 | 2018-10-24 09:06:48 | announced | |
Publication List
Parker R, Vella LJ, Xavier D, Amirkhani A, Parker J, Cebon J, Molloy MP, Phosphoproteomic Analysis of Cell-Based Resistance to BRAF Inhibitor Therapy in Melanoma. Front Oncol, 5():95(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Phosphoproteomics, BRAF, Drug resistance, Kinases, Label free quantitation, Mass spectrometry |
Contact List
Mark Molloy |
contact affiliation | Associate Professor APAF Director Australian Proteome Analysis Facility (APAF) Dept. Chemistry and Biomolecular Sciences E8C310, Research Park Drive Macquarie University Sydney NSW 2109 Australia Secretary, Australasian Proteomics Society |
contact email | MMolloy@proteome.org.au |
lab head | |
Robert Parker |
contact affiliation | Centre for High Throughput Biology |
contact email | robert.rparker@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002079
- Label: PRIDE project
- Name: Phosphoproteomic analysis of cell-based resistance to BRAF inhibitor therapy in melanoma