PXD001711-1

PXD001711 is an original dataset announced via ProteomeXchange.

Title	Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localisation.
Description	The deubiquitylating enzyme OTUB1 is ubiquitously expressed and is known to target a multitude of substrates, both in the cytosol and nucleus. Here, we demonstrate that phosphorylation of OTUB1 by Casein kinase 2 (CK2) at Ser16 triggers its nuclear accumulation. CK2 phosphorylates OTUB1 at Ser16 in vitro. In cells, endogenous OTUB1 is phosphorylated at Ser16 and this is blocked by chemical and genetic ablation of CK2 activity. Whereas OTUB1 is detected mainly in the cytosol, OTUB1 phosphorylated at Ser16 is detected only in the nucleus. Inhibition of CK2 causes nuclear exclusion of OTUB1. Although phosphorylation of OTUB1 at Ser16 does not alter its catalytic activity, ability to bind K63-linked ubiquitin chains and ability to interact with the E2 enzyme UBE2N in vitro, the nuclear localisation of OTUB1 appears to be essential for IR-induced DNA-damage repair.
HostingRepository	PRIDE
AnnounceDate	2015-04-15
AnnouncementXML	Submission_2015-04-15_05:24:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gopal Sapkota
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-21 01:20:17	ID requested
⏵ 1	2015-04-15 05:24:30	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: OTUB1, Casein kinase, CK2, phosphorylation, ubiquitin, DUB

Gopal P Sapkota
contact affiliation	MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dundee, United Kingdom
contact email	g.sapkota@dundee.ac.uk
lab head
Gopal Sapkota
contact affiliation	University of Dundee
contact email	g.sapkota@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001711
     1. Label: PRIDE project
     2. Name: Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localisation.