PXD001692 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein Structure Determination in Biological Matrices by Mass Spectrometry and Computational Biology |
Description | We have implemented the use of a heterobifunctional, UV photoactivatable cross-linker, which greatly increases the number of identified cross-links compared with homobifunctional, NHS-ester based cross-linkers. We have cross-linked human serum albumin in the context of human blood serum. We present a novel methodology that combines the use of this high-resolution cross-linking with conformational space search to investigate the structure of proteins in their native environment. |
HostingRepository | PRIDE |
AnnounceDate | 2015-09-22 |
AnnouncementXML | Submission_2015-09-22_04:30:40.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adam Belsom |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-01-19 01:18:12 | ID requested | |
⏵ 1 | 2015-09-22 04:30:41 | announced | |
2 | 2024-10-22 04:22:21 | announced | 2024-10-22: Updated project metadata. |
Publication List
Belsom A, Schneider M, Fischer L, Brock O, Rappsilber J, Serum Albumin Domain Structures in Human Blood Serum by Mass Spectrometry and Computational Biology. Mol Cell Proteomics, 15(3):1105-16(2016) [pubmed] |
Keyword List
curator keyword: Biological, Technical |
submitter keyword: Cross-linking and mass spectrometry, Human serum albumin |
Contact List
Juri Rappsilber |
contact affiliation | Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, United Kingdom. Department of Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany. |
contact email | juri.rappsilber@ed.ac.uk |
lab head | |
Adam Belsom |
contact affiliation | University of Edinburgh |
contact email | adam.belsom@ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/09/PXD001692 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001692
- Label: PRIDE project
- Name: Protein Structure Determination in Biological Matrices by Mass Spectrometry and Computational Biology