PXD001637-1

PXD001637 is an original dataset announced via ProteomeXchange.

Title	Breast cancer metastases .
Description	We have analysed a unique tumour set of fourteen primary breast cancer tumours with matched synchronous axillary lymph node metastases and a set of nine primary tumours with, later developed, matched distant metastases spread to different sites in the body. The aim was to further understand the molecular changes during the spreading and identify differentially regulated proteins that may be novel biomarkers or treatment targets. We analysed the changes in glycoprotein expression since protein glycosylation is predominant in both membrane proteins and secreted proteins and these proteins are often important for cancer transformation. This may also allow affinity capture enabling selected reaction monitoring of these biomarkers in blood. Glycopeptide capture was used in this study to selectively isolate and quantify N-linked glycopeptides from tumours mixtures and the captured glycopeptides were subjected to label-free quantitative tandem mass spectrometry analysis. Differentially expressed proteins between primary tumours and matched lymph node metastasis and distant metastasis were identified. Two of the top hits, ATPIF1 and tubulin β-chain were validated to be differentially regulated with immunohistochemistry.
HostingRepository	PRIDE
AnnounceDate	2024-10-03
AnnouncementXML	Submission_2024-10-03_07:59:01.229.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter James
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-02 01:44:57	ID requested
⏵ 1	2024-10-03 07:59:01	announced

Dataset with its publication pending

ProteomeXchange project tag: Bioinformatics Infrastructure for Life Sciences (BILS) network (Sweden)

curator keyword: Biomedical

submitter keyword: Cancer, Glycocapture,Breast

Peter James
contact affiliation	Immunotechnology Lund University
contact email	peter.james@immun.lth.se
lab head
Peter James
contact affiliation	Lund University
contact email	peter.james@immun.lth.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001637
     1. Label: PRIDE project
     2. Name: Breast cancer metastases