PXD001618-2

PXD001618 is an original dataset announced via ProteomeXchange.

Title	Phosphatase POPX2 secretome SILAC
Description	Cancer metastasis is a complex mechanism involving multiple processes. In an earlier study, we reported that the levels of serine/threonine phosphatase POPX2 were positively correlated with cancer cell motility through modulating MAPK signaling. Surprisingly, here we found that POPX2 knockdown cells induced more numerous and larger tumor nodules in lungs in longer term animal studies, suggesting that lower levels of POPX2 may favor tumor progression in later stages of metastasis. We hypothesize that POPX2 may do so by modulation of angiogenesis. Secretome analysis of POPX2-knockdown MDA-MB-231 cells using SILAC-mass spectrometry and cytokine array showed that silencing of POPX2 leads to increased secretion of exosomes, which may in turn induce multiple pro-angiogenic cytokines. This study, combined with our earlier findings, suggests that a single ubiquitously expressed phosphatase POPX2 influences cancer metastasis via modulating multiple biological processes including MAPK signaling and exosome-cytokine secretion.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:22:05.261.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tiannan Guo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-16 03:30:40	ID requested
1	2019-10-21 00:55:10	announced
⏵ 2	2024-10-22 04:22:05	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: POPX2 SILAC secretome

Newman Sze
contact affiliation	Nanyang Technological University
contact email	sksze@ntu.edu.sg
lab head
Tiannan Guo
contact affiliation	Westlake University
contact email	tiannan.guo@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001618
     1. Label: PRIDE project
     2. Name: Phosphatase POPX2 secretome SILAC