PXD001610-1

PXD001610 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of the Response to Cell Cycle Arrests in Human Myeloid Leukemia Cells
Description	Previously (Ly et al. 2014), we analyzed protein abundance changes across a ‘minimally perturbed’ cell cycle in human cells (NB4), using centrifugal elutriation to differentially enrich distinct cell cycle phases. Here, we compare data from elutriated cells with NB4 cells arrested at comparable phases using serum starvation, hydroxyurea, or RO-3306. While elutriated and arrested cells have similar patterns of DNA content and cyclin expression, a large fraction of the proteome changes detected in arrested cells are found to reflect arrest-specific responses (i.e., starvation, DNA damage, CDK1 inhibition), rather than physiological cell cycle regulation. For example, we show most cells arrested in G2 by CDK1 inhibition express abnormally high levels of replication and origin licensing factors, and are likely poised for genome re-replication. The protein data are available in the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/), an online, searchable resource.
HostingRepository	PRIDE
AnnounceDate	2015-07-23
AnnouncementXML	Submission_2015-07-23_04:26:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vackar Afzal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-15 05:50:51	ID requested
⏵ 1	2015-07-23 04:27:00	announced
2	2024-10-22 04:22:07	announced	2024-10-22: Updated project metadata.

Ly T, Endo A, Lamond AI, Proteomic analysis of the response to cell cycle arrests in human myeloid leukemia cells. Elife, 4():(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, LC-MSMS,

Angus Lamond
contact affiliation	Principle Investigator Proteomics
contact email	a.i.lamond@dundee.ac.uk
lab head
Vackar Afzal
contact affiliation	School of Life Sciences
contact email	v.z.afzal@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001610
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of the Response to Cell Cycle Arrests in Human Myeloid Leukemia Cells