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PXD001560 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA phosphoproteomic comparison of B-RAFV600E and MKK1/2 inhibitors in melanoma cells, part 1
DescriptionTo provide insight into the molecular nature of the clinical responses seen with MAPK pathway inhibition in melanoma, we used quantitative mass spectrometry to characterize the inhibitor-dependent phosphoproteome of human melanoma cells treated with the B-RAFV600E inhibitor PLX4032 (vemurafenib) and the MKK1/2 inhibitor AZD6244 (selumetinib).. In three replicate experiments, we quantified a total of 23,986 phosphosites on 4,722 proteins. This included 1,317 phosphosites that reproducibly decreased in response to at least one inhibitor.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterScott Stuart
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; phosphorylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-12-02 02:19:44ID requested
12016-02-25 06:15:30announced
22018-01-02 02:32:52announcedUpdated publication reference for PubMed record(s): 25850435, 29255136.
Publication List
Stuart SA, Houel S, Lee T, Wang N, Old WM, Ahn NG, A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics, 14(6):1599-615(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Human, Melanoma, SILAC, PLX4032, AZD6244
Contact List
Natalie G. Ahn
contact affiliationDepartment of Chemistry and Biochemistry University of Colorado, Boulder Boulder, CO 80309
contact emailNatalie.Ahn@colorado.edu
lab head
Scott Stuart
contact affiliationUC Boulder
contact emailscott.stuart@colorado.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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