PXD001560-1

PXD001560 is an original dataset announced via ProteomeXchange.

Title	A phosphoproteomic comparison of B-RAFV600E and MKK1/2 inhibitors in melanoma cells, part 1
Description	To provide insight into the molecular nature of the clinical responses seen with MAPK pathway inhibition in melanoma, we used quantitative mass spectrometry to characterize the inhibitor-dependent phosphoproteome of human melanoma cells treated with the B-RAFV600E inhibitor PLX4032 (vemurafenib) and the MKK1/2 inhibitor AZD6244 (selumetinib).. In three replicate experiments, we quantified a total of 23,986 phosphosites on 4,722 proteins. This included 1,317 phosphosites that reproducibly decreased in response to at least one inhibitor.
HostingRepository	PRIDE
AnnounceDate	2016-02-25
AnnouncementXML	Submission_2016-02-25_06:15:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Scott Stuart
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-02 02:19:44	ID requested
⏵ 1	2016-02-25 06:15:30	announced
2	2018-01-02 02:32:52	announced	Updated publication reference for PubMed record(s): 25850435, 29255136.
3	2024-10-22 04:23:43	announced	2024-10-22: Updated project metadata.

Stuart SA, Houel S, Lee T, Wang N, Old WM, Ahn NG, A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics, 14(6):1599-615(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Melanoma, SILAC, PLX4032, AZD6244

Natalie G. Ahn
contact affiliation	Department of Chemistry and Biochemistry University of Colorado, Boulder Boulder, CO 80309
contact email	Natalie.Ahn@colorado.edu
lab head
Scott Stuart
contact affiliation	UC Boulder
contact email	scott.stuart@colorado.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD001560

PRIDE project URI

• PRIDE
  1. PXD001560
     1. Label: PRIDE project
     2. Name: A phosphoproteomic comparison of B-RAFV600E and MKK1/2 inhibitors in melanoma cells, part 1