PXD001552 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of Metarhizium robertsii Lipid Droplets |
Description | Metarhizium robertsii is one of the model fungus species widely used studying insect-fungus interactions. Our previous studies have shown the accumulation of lipid droplets (LDs) play an essential role in generation of cellular turgor pressure to assist fungal penetration of insect cuticles, and autophagy-related proteins are connected with LD biogenesis and cellular accumulation in M. robertsii. However, full proteomes of LDs are unclear in terms of the species of proteins involved in lipid biosynthesis and degradation. We performed experiments by growing the fungi in different nutrient conditions, isolated the LDs and extracted the LD proteins from the cultures after being grown in a nutrient-rich medium (i.e. Sabouraud dextrose broth, SDB), a minimum medium without nitrogen source (MM-N) and MM-N supplemented with oleate. The protein samples were then subject to LC-MS/MS proteome profilings for identifying and postulating the proteins/pathways involved in lipid metabolisms. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:48:37.391.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chengshu Wang |
SpeciesList | scientific name: Metarhizium robertsii; NCBI TaxID: 568076; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-11-28 01:12:18 | ID requested | |
1 | 2022-03-01 06:51:46 | announced | |
⏵ 2 | 2023-11-14 08:48:37 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Metarhizium robertsii, Lipid Droplets, LC-MS/MS |
Contact List
Chengshu Wang |
contact affiliation | Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, China |
contact email | cswang@sibs.ac.cn |
lab head | |
Chengshu Wang |
contact affiliation | Shanghai Institute of Plant Physiology and Ecology, CAS |
contact email | cswang@sibs.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001552
- Label: PRIDE project
- Name: Proteomic analysis of Metarhizium robertsii Lipid Droplets