PXD001524-1

PXD001524 is an original dataset announced via ProteomeXchange.

Title	Human testicular matrix
Description	ITo derive a scaffold from human testis for the purpose of tissue engineering and regenerative medicine, we developed a method to produce a cytocompatible decellularized testicular matrix (DTM) while maintaining the native tissue-specific characteristics and components. The potential benefits of tissue-specific scaffolds consisting of naturally-derived extracellular matrix (ECM) have been demonstrated using a wide variety of animal and human tissue sources. However, so far, testis scaffolds have never been considered for constructive remodelling purposes. We have therefore developed a protocol for the preparation of cell-free extracellular matrix and characterized the material extensively using a combination of proteomics, immunohistochemsitry, and cell population assays. To prepare cell-free matrix, human cadaveric testicular tissue was exposed for 24 h or 48 h to 1% Triton X-100 and/or 1% sodium dodecyl sulfate (SDS). The extent of decellularization was evaluated by histology. Confirmation of cell removal in DTM was done by a DNA quantification technique. The protein composition was analysed by LC-MS/MS. The retention of testicular tissue-specific characteristics was evaluated by immunohistochemistry, Alcian blue staining and scanning electron microscopy. Soluble toxicity and testicular cell attachment was assessed to check the cytocompatibility of DTM scaffolds. Histological analysis showed that DTM could be obtained by mechanical agitation in 1% SDS for 24 h. The resulting DTM was found to be clear of cells while retaining the typical three-dimensional structure. Proteomics analysis revelaed the presence of the major components of the native tissue scaffold, including collagen type I and IV, fibronectin, laminin and glycosaminoglycans. In addition, numerous additional ECM proteins in DTM were detected, indicating its complex nature. Importantly, we demonstrated that DTM scaffolds are not cytotoxic, as evidenced by MTT assay showing a normal fibroblast proliferation activity after indirect exposure, and support testicular cell attachment and infiltration.
HostingRepository	PRIDE
AnnounceDate	2015-01-30
AnnouncementXML	Submission_2015-01-30_02:59:16.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001524
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Landreh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl; Deamidated
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-20 07:42:57	ID requested
⏵ 1	2015-01-30 02:59:18	announced

Baert Y, Stukenborg JB, Landreh M, De Kock J, J, ö, rnvall H, S, ö, der O, Goossens E, Derivation and characterization of a cytocompatible scaffold from human testis. Hum Reprod, 30(2):256-67(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Extracellular matrix, 3D cell culture, testicular structure

Hans Jornvall
contact affiliation	Medical Biochemistry and Biophysics, karolinska Institutet, Stockholm, Sweden
contact email	hans.jornvall@ki.se
lab head
Michael Landreh
contact affiliation	University of Oxford
contact email	michael.landreh@chem.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/01/PXD001524

PRIDE project URI

• PRIDE
  1. PXD001524
     1. Label: PRIDE project
     2. Name: Human testicular matrix