PXD001424 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The proteome of human iris |
Description | The iris is a fine structure that controls the amount of light that enters the eye. Iris diseases that result in visual disability and blindness include iritis, primary angle closure glaucoma, and pigment dispersion syndrome. A detailed investigation of the proteome of the normal human iris may provide a foundation for new investigations into the iris biology and pathophysiology. We conducted an in-depth proteomic analysis of five normal irides. Proteins were fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. We identified 3,000 non-redundant proteins in the human iris, including 26 unambiguous protein isoforms. The proteins identified in the iris included numerous proteins involved in smooth muscle motility, melanosome development, extracellular matrix, and selenoproteins involved in redox signaling. The MS proteome database of the human iris may serve as a valuable resource for future investigations of the eye in health and disease. |
HostingRepository | PRIDE |
AnnounceDate | 2016-07-18 |
AnnouncementXML | Submission_2016-07-18_09:37:16.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pingbo Zhang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | deamidated residue; iodoacetamide derivatized residue; phosphorylated residue; carbamoylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-10-20 09:08:16 | ID requested | |
⏵ 1 | 2016-07-18 09:37:18 | announced | |
2 | 2024-10-22 04:02:43 | announced | 2024-10-22: Updated project metadata. |
Publication List
Zhang P, Kirby D, Dufresne C, Chen Y, Turner R, Ferri S, Edward DP, Van Eyk JE, Semba RD, Defining the proteome of human iris, ciliary body, retinal pigment epithelium, and choroid. Proteomics, 16(7):1146-53(2016) [pubmed] |
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
curator keyword: Biomedical |
submitter keyword: Complement, Eye, Iris, Melanosome, Proteome, Selenoprotein |
Contact List
Richard D. Semba |
contact affiliation | 1Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD |
contact email | rdsemba@jhmi.edu |
lab head | |
Pingbo Zhang |
contact affiliation | Johns Hopkins University |
contact email | pzhang7@jhmi.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001424
- Label: PRIDE project
- Name: The proteome of human iris