PXD001407-1

PXD001407 is an original dataset announced via ProteomeXchange.

Title	Identification of protein-protein interactions involving the eIF4E binding proteins Caf20p and Eap1p on yeast via affinity purification and LC-MSMS.
Description	There are multiple translational control pathways. These include pathways involving eIF4E binding proteins (4E-BPs), which inhibit translation by binding and sequestering the 5’ cap binding protein eIF4E away from its partner eIF4G. Saccharomyces cerevisiae has two 4E-BPs: Caf20p and Eap1p. Previous analyses had shown that each 4E-BP regulates different subsets of mRNAs. In order to assess whether binding different proteins caused their different regulatory role, we used tandem affinity purification followed by label-free mass spectrometry to compare the proteomes pulled-down with the TAP-tagged 4E-BPs, and the global proteome. These analyses point out that Caf20p and Eap1p share most interaction partners, including ribosomes, and that both bind several other RNA-binding proteins.
HostingRepository	PRIDE
AnnounceDate	2016-06-29
AnnouncementXML	Submission_2016-06-29_09:03:49.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001407
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Talavera
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Dionex instrument model; Mascot; Progenesis LC-MS; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-17 00:54:11	ID requested
⏵ 1	2016-06-29 09:03:52	announced

Castelli LM, Talavera D, Kershaw CJ, Mohammad-Qureshi SS, Costello JL, Rowe W, Sims PF, Grant CM, Hubbard SJ, Ashe MP, Pavitt GD, The 4E-BP Caf20p Mediates Both eIF4E-Dependent and Independent Repression of Translation. PLoS Genet, 11(5):e1005233(2015) [pubmed]

curator keyword: Biological

submitter keyword: Yeast, Affinity purification, Label-free quantification, LC-MSMS

Graham Pavitt
contact affiliation	Faculty of Life Science, University of Manchester, United Kingdom
contact email	graham.pavitt@manchester.ac.uk
lab head
David Talavera
contact affiliation	University of Manchester
contact email	David.Talavera@manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001407
     1. Label: PRIDE project
     2. Name: Identification of protein-protein interactions involving the eIF4E binding proteins Caf20p and Eap1p on yeast via affinity purification and LC-MSMS.