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PXD001407 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of protein-protein interactions involving the eIF4E binding proteins Caf20p and Eap1p on yeast via affinity purification and LC-MSMS.
DescriptionThere are multiple translational control pathways. These include pathways involving eIF4E binding proteins (4E-BPs), which inhibit translation by binding and sequestering the 5’ cap binding protein eIF4E away from its partner eIF4G. Saccharomyces cerevisiae has two 4E-BPs: Caf20p and Eap1p. Previous analyses had shown that each 4E-BP regulates different subsets of mRNAs. In order to assess whether binding different proteins caused their different regulatory role, we used tandem affinity purification followed by label-free mass spectrometry to compare the proteomes pulled-down with the TAP-tagged 4E-BPs, and the global proteome. These analyses point out that Caf20p and Eap1p share most interaction partners, including ribosomes, and that both bind several other RNA-binding proteins.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDavid Talavera
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentDionex instrument model; Mascot; Progenesis LC-MS; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-10-17 00:54:11ID requested
12016-06-29 09:03:52announced
Publication List
Castelli LM, Talavera D, Kershaw CJ, Mohammad-Qureshi SS, Costello JL, Rowe W, Sims PF, Grant CM, Hubbard SJ, Ashe MP, Pavitt GD, The 4E-BP Caf20p Mediates Both eIF4E-Dependent and Independent Repression of Translation. PLoS Genet, 11(5):e1005233(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Yeast, Affinity purification, Label-free quantification, LC-MSMS
Contact List
Graham Pavitt
contact affiliationFaculty of Life Science, University of Manchester, United Kingdom
contact emailgraham.pavitt@manchester.ac.uk
lab head
David Talavera
contact affiliationUniversity of Manchester
contact emailDavid.Talavera@manchester.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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