PXD001357-1

PXD001357 is an original dataset announced via ProteomeXchange.

Title	Direct evidence of milk consumption from ancient human dental calculus, St Helena
Description	This study investigated the consumption of milk products in the archaeological record, utilizing human dental calculus as a reservoir of dietary proteins from archaeological samples from across Eurasia. Protein extraction and generation of tryptic peptides from dental calculus was performed using a filter-aided sample preparation (FASP) protocol, modified for ancient samples, on 92 samples of archaeological dental calculus. Samples were extracted at three laboratories; the Functional Genomics Centre Zürich (FGCZ), the Centre for GeoGenetics at the National History Museum of Denmark, and BioArCh at the University of York. Sample extracts were sequenced (LC-MS/MS) using an LTQ-Orbitrap Velos (FGCZ), a Q-Exactive Hybrid Quadrupole Orbitrap and an LTQ-Orbitrap Elite (Central Proteomics Facility, Target Discovery Institute, Oxford).
HostingRepository	PRIDE
AnnounceDate	2015-02-25
AnnouncementXML	Submission_2015-02-25_04:42:02.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001357
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jessica Hendy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Gln->pyro-Glu; Oxidation; Acetyl; Carbamidomethyl; Deamidated
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-24 01:25:50	ID requested
⏵ 1	2015-02-25 04:42:03	announced

Warinner C, Hendy J, Speller C, Cappellini E, Fischer R, Trachsel C, Arneborg J, Lynnerup N, Craig OE, Swallow DM, Fotakis A, Christensen RJ, Olsen JV, Liebert A, Montalva N, Fiddyment S, Charlton S, Mackie M, Canci A, Bouwman A, R, ü, hli F, Gilbert MT, Collins MJ, Direct evidence of milk consumption from ancient human dental calculus. Sci Rep, 4():7104(2014) [pubmed]

curator keyword: Metaproteomics, Technical

submitter keyword: Human, Dental Calculus, Dental Plaque, LC-MS/MS, Beta-lactoglobulin

Matthew Collins
contact affiliation	BioArCh, University of York, UK
contact email	matthew.collins@york.ac.uk
lab head
Jessica Hendy
contact affiliation	University of York
contact email	jessica.hendy@york.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/02/PXD001357

PRIDE project URI

• PRIDE
  1. PXD001357
     1. Label: PRIDE project
     2. Name: Direct evidence of milk consumption from ancient human dental calculus, St Helena