PXD001348-1

PXD001348 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of the effect of deletion and mutation of eIF4E binding proteins Caf20 and Eap1 on yeast via LC-MSMS.
Description	There are multiple translational control pathways. These include pathways involving eIF4E binding proteins (4E-BPs), which inhibit translation by binding and sequestering the 5’ cap binding protein eIF4E away from its partner eIF4G. Saccharomyces cerevisiae has two 4E-BPs: Caf20p and Eap1p; and, microarray analysis has shown that each regulates different subsets of mRNAs. To assess the effect of these different regulatory responses at the protein level, we used label-free mass spectrometry to compare the proteomes of wild-type strain with those of caf20Δ and eap1Δ single mutant strains; caf20Δ and eap1Δ double mutant strain; and, caf20Δ mutant strain transformed with one plasmid containing a mutated version of caf20. These analyses indicate that Caf20p and Eap1p may compensate for each other loss, and also reveal that Caf20p participates in translational control pathways that are independent of eIF4E binding.
HostingRepository	PRIDE
AnnounceDate	2016-05-04
AnnouncementXML	Submission_2016-05-04_08:01:24.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001348
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Talavera
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Dionex instrument model; Mascot; Progenesis LC-MS; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-19 07:47:41	ID requested
⏵ 1	2016-05-04 08:01:25	announced

Castelli LM, Talavera D, Kershaw CJ, Mohammad-Qureshi SS, Costello JL, Rowe W, Sims PF, Grant CM, Hubbard SJ, Ashe MP, Pavitt GD, The 4E-BP Caf20p Mediates Both eIF4E-Dependent and Independent Repression of Translation. PLoS Genet, 11(5):e1005233(2015) [pubmed]

curator keyword: Biological

submitter keyword: Yeast, Label-free quantification, LC-MSMS

Graham Pavitt
contact affiliation	Faculty of Life Science, University of Manchester, United Kingdom
contact email	graham.pavitt@manchester.ac.uk
lab head
David Talavera
contact affiliation	University of Manchester
contact email	David.Talavera@manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001348
     1. Label: PRIDE project
     2. Name: Proteomics analysis of the effect of deletion and mutation of eIF4E binding proteins Caf20 and Eap1 on yeast via LC-MSMS.