PXD001339-2

PXD001339 is an original dataset announced via ProteomeXchange.

Title	Huh-7 exosomes and HBV
Description	Hepatitis B virus (HBV) infection could cause hepatitis, liver cirrhosis and hepatocellular carcinoma. HBV-mediated pathogenesis is only partially understood, but X protein (HBx) reportedly possesses oncogenic potential. Exosomes are small membrane vesicles with diverse functions released by various cells including hepatocytes, and HBV harnesses cellular exosome biogenesis and export machineries for virion morphogenesis and secretion. Therefore, HBV infection might cause changes in exosome contents with functional implications for both virus and host. In this project, exosome protein content changes induced by HBV and HBx were quantitatively analyzed by SILAC/LC-MS/MS. Exosomes prepared from SILAC-labeled hepatoma cell line Huh-7 transfected with HBx, wildtype or HBx-null HBV replicon plasmids were analyzed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2014-10-09
AnnouncementXML	Submission_2014-10-10_02:04:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-17 08:22:53	ID requested
1	2014-10-09 08:00:21	announced
⏵ 2	2014-10-10 02:04:38	announced	Updated publication reference for PubMed record(s): 25265333.
3	2024-10-22 03:59:09	announced	2024-10-22: Updated project metadata.

Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J, Quantitative proteomic analysis of exosome protein content changes induced by hepatitis B virus in Huh-7 cells using SILAC labeling and LC-MS/MS. J Proteome Res, 13(12):5391-402(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Hepatitis B virus, HBx, exosome

Youhua Xie
contact affiliation	Key Laboratory of Medical Molecular Virology (MOE/MOH) and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University
contact email	yhxie@fudan.edu.cn
lab head
Jing Liu
contact affiliation	Fudan University
contact email	liujing212@fudan.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/10/PXD001339

PRIDE project URI

• PRIDE
  1. PXD001339
     1. Label: PRIDE project
     2. Name: Huh-7 exosomes and HBV