PXD001159-2

PXD001159 is an original dataset announced via ProteomeXchange.

Title	Identification of immunoreactive proteins of Mycobacterium avium subsp. Paratuberculosis
Description	Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of a chronic enteritis of ruminants (bovine paratuberculosis-Johne disease) that is associated with enormous worldwide economic losses for the zootechnical industries. Diagnosis is based on observation of clinical signs, on the detection of antibodies in milk or serum or on evaluation of bacterial culture from feces. The limit of these methods is that they are not able to detect the disease in the subclinical stage and are applicable only when the disease is already in an advanced status. For this reason the main purpose of this study is to use the MAP proteome to detect novel immunoreactive proteins that may be helpful for paratuberculosis diagnoses. 2D electrophoresis and 2D immunoblotting of MAP proteins were performed using sera of control cattle and paratuberculosis infected cattle in order to highlight the specific immunoreactive proteins. Among the assigned identifiers to immunoreactive spots it was found that most of them correspond to surface-located proteins while three of them have never been described before as antigens. The identification of these proteins improves scientific knowledge that could be useful for paratuberculosis diagnoses. The sequence of the identified protein can be used for the synthesis of immunoreactive peptides that could be screened for their immunoreaction against bovine sera infected with MAP.
HostingRepository	PRIDE
AnnounceDate	2017-08-02
AnnouncementXML	Submission_2017-08-02_05:47:46.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001159
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paola Roncada
SpeciesList	scientific name: Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10); NCBI TaxID: 262316; scientific name: Other Actinobacteria (class); NCBI TaxID: 1760;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-18 07:26:58	ID requested
1	2014-11-20 03:26:28	announced
⏵ 2	2017-08-02 05:47:47	announced	Updated project metadata.
3	2024-10-22 04:06:47	announced	2024-10-22: Updated project metadata.

Piras C, Soggiu A, Bonizzi L, Greco V, Ricchi M, Arrigoni N, Bassols A, Urbani A, Roncada P, Identification of immunoreactive proteins of Mycobacterium avium subsp. paratuberculosis. Proteomics, 15(4):813-23(2015) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mycobacterium avium subsp. paratuberculosis

Johne’s disease

immunoreactive proteins

soil biological contaminant

Dr Paola Roncada
contact affiliation	1. Department of Veterinary Science and Public Health (DIVET), University of Milan 2. Istituto Sperimentale Italiano Lazzaro Spallanzani, Milano
contact email	paola.roncada@guest.unimi.it
lab head
Paola Roncada
contact affiliation	DIVET, Università degli studi di Milano
contact email	paola.roncada@guest.unimi.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/11/PXD001159

PRIDE project URI

• PRIDE
  1. PXD001159
     1. Label: PRIDE project
     2. Name: Identification of immunoreactive proteins of Mycobacterium avium subsp. Paratuberculosis