PXD001129-1

PXD001129 is an original dataset announced via ProteomeXchange.

Title	Dissection of IL-2 and IL-15 signaling pathways
Description	The aim of this study was to compare the signaling cascades initiated by the two closely related cytokines IL-2 and IL-15. Considering the relevance of protein tyrosine phosphorylation in signal transduction, in order to quantify changes in protein phosphorylation in response to IL-2 and IL-15, we combined triple SILAClabeling of leukemic T-cells with phosphotyrosine (pY)-specific antibody-based protein enrichment followed by mass spectrometry (MS) analysis. Following the strategy described above, we managed to decipher in detail the complex signaling networks triggered by IL-2 and IL-15. Interestingly, a large number of components of the three main signaling pathways known to be initiated in response to the interleukins (JAK/STAT; RAS/MAPK; PI3K/AKT) were identified.
HostingRepository	PRIDE
AnnounceDate	2014-09-23
AnnouncementXML	Submission_2014-09-23_07:41:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nerea Osinalde
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-11 03:23:51	ID requested
⏵ 1	2014-09-23 07:41:46	announced
2	2024-10-22 04:05:36	announced	2024-10-22: Updated project metadata.

Osinalde N, Sanchez-Quiles V, Akimov V, Guerra B, Blagoev B, Kratchmarova I, Simultaneous dissection and comparison of IL-2 and IL-15 signaling pathways by global quantitative phosphoproteomics. Proteomics, 15(2-3):520-31(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: SILAC, signaling, phosphotyrosine, IL-2, IL-15

Nerea Osinalde
contact affiliation	Department of Biochemistry and Molecular Biology, Center of Experimental BioInformatic, University of Southern Denmark
contact email	nmo@bmb.sdu.dk
lab head
Nerea Osinalde
contact affiliation	Postdoc
contact email	nmo@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/09/PXD001129

PRIDE project URI

• PRIDE
  1. PXD001129
     1. Label: PRIDE project
     2. Name: Dissection of IL-2 and IL-15 signaling pathways