PXD001129 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dissection of IL-2 and IL-15 signaling pathways |
Description | The aim of this study was to compare the signaling cascades initiated by the two closely related cytokines IL-2 and IL-15. Considering the relevance of protein tyrosine phosphorylation in signal transduction, in order to quantify changes in protein phosphorylation in response to IL-2 and IL-15, we combined triple SILAClabeling of leukemic T-cells with phosphotyrosine (pY)-specific antibody-based protein enrichment followed by mass spectrometry (MS) analysis. Following the strategy described above, we managed to decipher in detail the complex signaling networks triggered by IL-2 and IL-15. Interestingly, a large number of components of the three main signaling pathways known to be initiated in response to the interleukins (JAK/STAT; RAS/MAPK; PI3K/AKT) were identified. |
HostingRepository | PRIDE |
AnnounceDate | 2014-09-23 |
AnnouncementXML | Submission_2014-09-23_07:41:45.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nerea Osinalde |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-07-11 03:23:51 | ID requested | |
⏵ 1 | 2014-09-23 07:41:46 | announced | |
Publication List
Osinalde N, Sanchez-Quiles V, Akimov V, Guerra B, Blagoev B, Kratchmarova I, Simultaneous dissection and comparison of IL-2 and IL-15 signaling pathways by global quantitative phosphoproteomics. Proteomics, 15(2-3):520-31(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: SILAC, signaling, phosphotyrosine, IL-2, IL-15 |
Contact List
Nerea Osinalde |
contact affiliation | Department of Biochemistry and Molecular Biology, Center of Experimental BioInformatic, University of Southern Denmark |
contact email | nmo@bmb.sdu.dk |
lab head | |
Nerea Osinalde |
contact affiliation | Postdoc |
contact email | nmo@bmb.sdu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/09/PXD001129 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001129
- Label: PRIDE project
- Name: Dissection of IL-2 and IL-15 signaling pathways