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PXD001129 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDissection of IL-2 and IL-15 signaling pathways
DescriptionThe aim of this study was to compare the signaling cascades initiated by the two closely related cytokines IL-2 and IL-15. Considering the relevance of protein tyrosine phosphorylation in signal transduction, in order to quantify changes in protein phosphorylation in response to IL-2 and IL-15, we combined triple SILAClabeling of leukemic T-cells with phosphotyrosine (pY)-specific antibody-based protein enrichment followed by mass spectrometry (MS) analysis. Following the strategy described above, we managed to decipher in detail the complex signaling networks triggered by IL-2 and IL-15. Interestingly, a large number of components of the three main signaling pathways known to be initiated in response to the interleukins (JAK/STAT; RAS/MAPK; PI3K/AKT) were identified.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNerea Osinalde
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-07-11 03:23:51ID requested
12014-09-23 07:41:46announced
Publication List
Osinalde N, Sanchez-Quiles V, Akimov V, Guerra B, Blagoev B, Kratchmarova I, Simultaneous dissection and comparison of IL-2 and IL-15 signaling pathways by global quantitative phosphoproteomics. Proteomics, 15(2-3):520-31(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: SILAC, signaling, phosphotyrosine, IL-2, IL-15
Contact List
Nerea Osinalde
contact affiliationDepartment of Biochemistry and Molecular Biology, Center of Experimental BioInformatic, University of Southern Denmark
contact emailnmo@bmb.sdu.dk
lab head
Nerea Osinalde
contact affiliationPostdoc
contact emailnmo@bmb.sdu.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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