PXD001115-2

PXD001115 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomics of NGF-TrkA signaling dynamics in neuroblastoma cells
Description	We used quantitative mass spectrometry-based proteomics to unravel global nerve growth factor (NGF)-induced TrkA signaling dynamics at the interactome, phosphoproteome and proteome level. A tetracycline-inducible system for TrkA expression was generated in the human neuroblastoma cell line, SH-SY5Y. TrkA-induced cells were stimulated with NGF for different time points to follow phosphoproteome, interactome and proteome changes on a temporal scale. In a triple SILAC setup (Light: Lys0,Arg0; Medium: Lys4,Arg6; and Heavy: Lys8,Arg10), the samples were stimulated with NGF as indicated. Phosphoproteome: 0, 10, 45 min and 0, 120, 120 min+cycloheximide. Interactome: 0, 5, 10 min. Proteome: 0, 24, 48 h. All experiments were performed as biological replicates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:19.628.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristina Bennet Emdal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-08 07:10:46	ID requested
1	2015-05-06 07:44:41	announced
⏵ 2	2024-10-22 04:05:27	announced	2024-10-22: Updated project metadata.

Publication List

10.1126/scisignal.2005769;
Emdal KB, Pedersen AK, Bekker-Jensen DB, Tsafou KP, Horn H, Lindner S, Schulte JH, Eggert A, Jensen LJ, Francavilla C, Olsen JV, Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation. Sci Signal, 8(374):ra40(2015) [pubmed]

10.1126/scisignal.2005769;

Emdal KB, Pedersen AK, Bekker-Jensen DB, Tsafou KP, Horn H, Lindner S, Schulte JH, Eggert A, Jensen LJ, Francavilla C, Olsen JV, Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation. Sci Signal, 8(374):ra40(2015) [pubmed]

Keyword List

curator keyword: Biomedical
submitter keyword: neuroblastoma, phosphoproteome, mass spectrometry, SILAC,NGF, proteome, TrkA, interactome

curator keyword: Biomedical

submitter keyword: neuroblastoma, phosphoproteome, mass spectrometry, SILAC,NGF, proteome, TrkA, interactome

Contact List

Jesper V. Olsen
contact affiliation	Novo Nordisk Foundation Center for Protein Research Faculty of Health & Medical Sciences University of Copenhagen Blegdamsvej 3B DK-2200 Copenhagen N Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Kristina Bennet Emdal
contact affiliation	NNF Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen.
contact email	kristina.emdal@cpr.ku.dk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/05/PXD001115

PRIDE project URI

Repository Record List

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