PXD001056 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Label free IMS-MSE protein quantification for plant Golgi protein localisation and abundance |
Description | Golgi resident proteins in Arabidopsis thaliana are challenging to quantify since the abundance of this organelle is relatively low within the cell. In this study an organelle fractionation approach, targeting the Golgi apparatus, was combined with a label free quantitative MS, data-independent acquisition (DIA) method employing ion mobility separation known as LC-IMS-MSE, to simultaneously localise proteins to the Golgi apparatus and assess their relative quantity. In total 33 proteins were localised to Golgi apparatus and 102 Golgi localised proteins were quantified. |
HostingRepository | PRIDE |
AnnounceDate | 2016-07-08 |
AnnouncementXML | Submission_2016-07-08_06:18:21.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pavel Shliaha |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-06-11 03:11:32 | ID requested | |
⏵ 1 | 2016-07-08 06:18:22 | announced | |
Publication List
Nikolovski N, Shliaha PV, Gatto L, Dupree P, Lilley KS, Label-free protein quantification for plant Golgi protein localization and abundance. Plant Physiol, 166(2):1033-43(2014) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Arabidopsis thaliana, organelle proteomics, absolute quantitation, LOPIT, LOPIMS, MSE, HDMSE, IMS-MSE, data-independent acquisition |
Contact List
Kathryn Susan Lilley |
contact affiliation | Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, UK |
contact email | ksl23@cam.ac.uk |
lab head | |
Pavel Shliaha |
contact affiliation | Cambridge Center For Proteomics |
contact email | pvs22@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001056
- Label: PRIDE project
- Name: Label free IMS-MSE protein quantification for plant Golgi protein localisation and abundance