PXD000987-2

PXD000987 is an original dataset announced via ProteomeXchange.

Title	Membrane proteomis profile along human colon
Description	The colonic epithelium is a highly dynamic system important for regulation of ion and water homeostasis via absorption and secretion, and in maintaining a protective barrier between the outer milieu and the inside of our body. These processes are known to gradually change along the length of the colon, although a complete characterization at the protein level is lacking. We therefore analyzed the membrane proteome of isolated human (n=4) colonic epithelial cells from biopsies obtained via routine colonoscopy for four segments along the large intestine: ascending, transverse, descending and sigmoid colon. Label-free quantitative proteomic analyses using high-resolution mass spectrometry were performed on enriched membrane proteins. The results showed a stable level for the majority of membrane proteins, while a distinct decrease in proteins associated with bacterial sensing, cation-transport and O-glycosylation in the proximal to distal direction. Proteins involved in microbial defense and anion-transport showed on the other hand an opposing gradient and increased towards the distal end. The gradient of ion-transporter proteins could be directly related to previously observed ion transport activities. All individual glycosyltransferases required for the O-glycosylation of the major colonic mucin MUC2 were observed and correlated with the known glycosylation variation along the colon axis. This is the first comprehensive quantitative dataset of membrane protein abundance along the human colon and will add to the knowledge of the physiological function of the different regions of the colonic mucosa.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_03:56:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	S.S van der Post
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-19 05:19:03	ID requested
1	2014-07-31 07:31:36	announced
⏵ 2	2014-10-23 03:56:02	announced	Updated project metadata.
3	2024-10-22 04:20:52	announced	2024-10-22: Updated project metadata.

van der Post S, Hansson GC, Membrane protein profiling of human colon reveals distinct regional differences. Mol Cell Proteomics, 13(9):2277-87(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, colon, label-free, LC/MS/MS

Gunnar C Hansson
contact affiliation	Department of Medical Biochemistry, University of Gothenburg
contact email	gunnar.hansson@medkem.gu.se
lab head
S.S van der Post
contact affiliation	Medical Chemistry
contact email	sjoerd.van.der.post@medkem.gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/07/PXD000987

PRIDE project URI

• PRIDE
  1. PXD000987
     1. Label: PRIDE project
     2. Name: Membrane proteomis profile along human colon