PXD000891 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Neisseria meningitides small RNA's regulate Citric acid cycle activity under Feast and Famine conditions |
Description | We identified twin small non-coding RNAs regulating tricarboxylic acid (TCA) cycle activity in Neisseria meningitidis. Expression of TCA cycle enzymes was elevated in sRNA deletion mutants. Direct interaction between sRNAs and the ribosomal entry sites on target mRNAs was demonstrated. Expression of the sRNAs was down-regulated in cells grown in poor medium with glucose as the sole carbon source but not without lrp, indicating that sRNA expression is controlled by the stringent response. N. meningitidis, over-expressing the sRNAs replicated in blood, but not in human cerebrospinal fluid. In addition, Lrp synthesis was inhibited by direct interaction between the sRNA and the 5’ UTR of lrp. sRNAs control adaptation of N. meningitidis to variation in nutrient supply in different niches of its host. |
HostingRepository | PRIDE |
AnnounceDate | 2017-03-22 |
AnnouncementXML | Submission_2017-03-27_01:31:27.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gertjan Kramer |
SpeciesList | scientific name: Neisseria meningitidis serogroup B; NCBI TaxID: 491; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-04-10 05:23:01 | ID requested | |
1 | 2017-03-22 05:07:05 | announced | |
⏵ 2 | 2017-03-27 01:31:28 | announced | Updated publication reference for PubMed record(s): 28325760. |
Publication List
Pannekoek Y, Huis In 't Veld RA, Schipper K, Bovenkerk S, Kramer G, Brouwer MC, van de Beek D, Speijer D, van der Ende A, Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism. mBio, 8(2):(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Neisseria meningitides, small RNA's, Feast and Famine, Data Independant Mass Spectrometry, Pathogens |
Contact List
Dave Speijer |
contact affiliation | Clinical Proteomics Facility Medical Biochemistry Academic Medical Center, University of Amsterdam |
contact email | d.speijer@amc.uva.nl |
lab head | |
Gertjan Kramer |
contact affiliation | Proteomics Unit |
contact email | gertjan.kramer@embl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000891
- Label: PRIDE project
- Name: Neisseria meningitides small RNA's regulate Citric acid cycle activity under Feast and Famine conditions