PXD000890-2

PXD000890 is an original dataset announced via ProteomeXchange.

Title	Decoding Listeria by N-terminomics
Description	This dataset contains spectral information of protein N-terminal peptides isolated from Listeria monocytogenes EGD-e, a bacterial model organism and human pathogen. When mapped onto the Listeria genome these peptides indicate the exact location of translation initiation sites (TIS). The large majority of the identified TIS corresponded to start sites of predicted open reading frames (ORFs), however, a significant fraction of the identified TIS indicated deviations from the current genome annotation. The latter include primarily TIS inside the sequence of predicted ORFs or TIS that delineate the start position of novel ORFs.
HostingRepository	PRIDE
AnnounceDate	2017-01-02
AnnouncementXML	Submission_2017-02-20_03:47:23.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000890
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Francis Impens
SpeciesList	scientific name: Listeria monocytogenes EGD; NCBI TaxID: 1334565;
ModificationList	acetate labeling reagent (N-term) (heavy form: +3amu); S-carboxamidomethyl-L-cysteine: 57.021464; N-formylated residue: 27.994915; N-acetylated residue: 42.010565; 3x(2)H labeled N6-acetyl-L-lysine: 45.029395; L-methionine (S)-sulfoxide: 15.994915
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-10 03:39:58	ID requested
1	2017-01-02 12:53:07	announced
⏵ 2	2017-02-20 03:47:24	announced	Updated publication reference for PubMed record(s): 28191904.

Impens F, Rolhion N, Radoshevich L, B, é, cavin C, Duval M, Mellin J, Garc, í, a Del Portillo F, Pucciarelli MG, Williams AH, Cossart P, N-terminomics identifies Prli42 as a membrane miniprotein conserved in Firmicutes and critical for stressosome activation in Listeria monocytogenes. Nat Microbiol, 2():17005(2017) [pubmed]

curator keyword: Biological

submitter keyword: Listeria, proteogenomics, N-terminal COFRADIC, translation initiation

Pascale Cossart
contact affiliation	Institut Pasteur Unité des Interactions Bactéries-Cellules, Inserm U604, INRA USC2020 25 rue du Dr. Roux 75015 Paris France
contact email	pascale.cossart@pasteur.fr
lab head
Francis Impens
contact affiliation	Institut Pasteur
contact email	francis.impens@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD000890

PRIDE project URI

• PRIDE
  1. PXD000890
     1. Label: PRIDE project
     2. Name: Decoding Listeria by N-terminomics