PXD000868-1

PXD000868 is an original dataset announced via ProteomeXchange.

Title	Large-scale phosphoproteome analysis in seedling leaves of Brachypodium distachyon L
Description	In this study, we carried out the first large-scale phosphoproteome analysis of seedling leaves in Brachypodium accession Bd21 using TiO2 microcolumns combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and MaxQuant software. A total, 1,470 phosphorylation sites in 950 phosphoproteins were identified. Of the 950 phosphoproteins identified, 127 contained 3 to 8 phosphorylation sites. The phosphoproteins and phosphosites identified in our study expanded our knowledge of protein phosphorylation modification in plants, especially in monocot.
HostingRepository	PRIDE
AnnounceDate	2016-07-09
AnnouncementXML	Submission_2016-07-08_17:18:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong-Wen Lv
SpeciesList	scientific name: Brachypodium distachyon (Purple false brome) (Trachynia distachya); NCBI TaxID: 15368;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-01 07:33:22	ID requested
⏵ 1	2016-07-08 17:18:14	announced
2	2024-10-22 04:20:19	announced	2024-10-22: Updated project metadata.

Lv DW, Li X, Zhang M, Gu AQ, Zhen SM, Wang C, Li XH, Yan YM, Large-scale phosphoproteome analysis in seedling leaves of Brachypodium distachyon L. BMC Genomics, 15(1):375(2014) [pubmed]

submitter keyword: Bd21, leaf, phosphoproteome, transcription factors, phosphorylation motif, protein kinases

Yue-Ming Yan
contact affiliation	College of Life Science, Capital Normal University, Beijing 100048, China
contact email	yanym@cnu.edu.cn
lab head
Dong-Wen Lv
contact affiliation	College of Life Sciences
contact email	dw.lv@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD000868

PRIDE project URI

• PRIDE
  1. PXD000868
     1. Label: PRIDE project
     2. Name: Large-scale phosphoproteome analysis in seedling leaves of Brachypodium distachyon L