PXD000699-1

PXD000699 is an original dataset announced via ProteomeXchange.

Title	Trichomonas vaginalis .
Description	Trichomonas vaginalis is a sexually transmitted infection that causes vaginitis and increases the risk of HIV transmission. We are interested in the secreted and membrane glycoproteins of Trichomonas because they are likely involved in pathogenesis and may include novel vaccine targets. Four mass spectrometric methods (identification of all parasite proteins, glycoprotein enrichment with the plant lectin Concanavalin A, peptide:N-glycanase treatment to identify occupied N-glycans sites, and analysis of N-terminal peptides) were used to identify >300 Trichomonas secreted and membrane proteins. The first group of these proteins, which were present in multiple genome copies and had homologs in diverse eukaryotes, included 1) those involved in the N-glycan-dependent quality control protein folding in the ER lumen, 2) metalloproteases, serine proteases, cysteine proteases, and other lysosomal enzymes, and 3) transporters and membrane-associated cyclases. The second group of secreted and membrane proteins were, for the most part, encoded by single copy genes, unique to Trichomonas, and missing N-terminal signal peptides. The latter observation is despite evidence that the signal peptide peptidase functions normally in Trichomonas. As the unique secreted and membrane proteins of Trichomonas were often large and lacked features that make it easy to choose vaccine candidates, alternative strategies for vaccination and/or therapy are discussed.
HostingRepository	PRIDE
AnnounceDate	2024-10-03
AnnouncementXML	Submission_2024-10-03_07:58:42.712.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000699
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Carpentieri
SpeciesList	scientific name: Eukaryota (eucaryotes); NCBI TaxID: 2759; scientific name: Trichomonas vaginalis; NCBI TaxID: 5722;
ModificationList	deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-01-21 06:13:11	ID requested
⏵ 1	2024-10-03 07:58:43	announced

10.6019/PXD000699;

Cui J, Smith TF, Samuelson J, Gene expansion in Trichomonas vaginalis: a case study on transmembrane cyclases. Genome Inform, 18():35-43(2007) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Trichomonas vaginalis, proteomics, Con-A, trichomoniasis

John Crawford Samuelson
contact affiliation	Boston University Henry M. Goldman School of Dental Medicine, Department of Molecular & Cell Biology. Boston University School of Medicine, Department of Microbiology
contact email	jsamuels@bu.edu
lab head
Andrea Carpentieri
contact affiliation	Chemical Sciences
contact email	acarpent@unina.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD000699

PRIDE project URI

• PRIDE
  1. PXD000699
     1. Label: PRIDE project
     2. Name: Trichomonas vaginalis