PXD000632-2

PXD000632 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	FLAG-affinity pulldown experiments with sll1214 and sll1874 from Synechocystis
Description	Putative subunits of the Mg-protoporphyrin monomethylester oxidative cyclase from Synechocystis PCC 6803, sll1214 and sll1874 were expressed with N-terminal 3xFLAG-tags in separate transformants.. Solubilised thylakoid membranes from these 2 transformants were incubated with anti-FLAG agarose and after extensive washing, the FLAG-tagged proteins, together with putative interaction partners were desorbed with FLAG peptide. The eluates were denatured, reduced, alkylated and digested with trypsin. The resulting peptides were cleaned by cation exchange and desalted, then analysed by nanoLC-MS/MS. The FLAG pulldown experiment was also carried out with a thylakoid membrane preparation from wild-type with no FLAG-tagged proteins as a control. Protein identification was by database searching with Mascot.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_09:15:27.971.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000632
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Synechocystis sp. (strain PCC 6803 / Kazusa); NCBI TaxID: 1111708;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	maXis II

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-10 04:02:12	ID requested
1	2015-08-05 04:20:17	announced
⏵ 2	2024-10-07 09:15:29	announced	2024-10-07: Updated project metadata.

Publication List

10.6019/PXD000632;
Hollingshead S, Kopecn, á J, Jackson PJ, Canniffe DP, Davison PA, Dickman MJ, Sobotka R, Hunter CN, Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803. J Biol Chem, 287(33):27823-33(2012) [pubmed]
10.1074/jbc.M112.352526;

10.6019/PXD000632;

Hollingshead S, Kopecn, á J, Jackson PJ, Canniffe DP, Davison PA, Dickman MJ, Sobotka R, Hunter CN, Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803. J Biol Chem, 287(33):27823-33(2012) [pubmed]

10.1074/jbc.M112.352526;

Keyword List

submitter keyword: sll1214, sll1874, Ycf54, Mg-protoporphyrin monomethylester oxidative cyclase, FLAG-tag, Maxis UHR-TOF,Synechocystis PCC 6803

submitter keyword: sll1214, sll1874, Ycf54, Mg-protoporphyrin monomethylester oxidative cyclase, FLAG-tag, Maxis UHR-TOF,Synechocystis PCC 6803

Contact List

Philip Jackson
contact affiliation	School of Biosciences, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
lab head
Philip Jackson
contact affiliation	School of Biosciences, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD000632

PRIDE project URI

Repository Record List

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