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PXD000533-1

PXD000533 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCHPP chr 1,8,20 proteome dataset using the HCC cell lines of Hep3B and MHCC97H, instrument is Q Exactive, part 2 of 3
DescriptionThe fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-solution protein digestion by Lys-C and trypsin at 37 degrees Celsius, respectively. The tryptic peptides were cleaned by C18 Sep-Pak column (Waters UK Ltd, Manchester, UK). The mass spectrometer used in this dataset was Q-Exactive. The dataset was produced with two technical replicates and 24 fractions per repeat. The database searching procedure was achieved using Mascot v2.3. The database is Swiss-prot (release 2013 -6).
HostingRepositoryPRIDE
AnnounceDate2013-12-19
AnnouncementXMLSubmission_2013-12-19_02:27:37.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000533
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterZhang Chengpu Zhang Chengpu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; neutral loss: 63.998285
Instrumentquadrupole
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-21 07:03:32ID requested
12013-12-19 02:27:54announced
22015-08-12 02:48:01announcedUpdated publication reference for PubMed record(s): 24328083.
32024-10-07 09:11:19announced2024-10-07: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: CHPP, chromesome 1,8,20, HCC cell lines, Q-Exactive
Contact List
Zhang Chengpu Zhang Chengpu
contact affiliationBeijing proteome research center
contact emailzhangchengpu0203@163.com
zcp
contact attribute448377867@qq.com
contact affiliationBPRC
contact email448377867@qq.com
Full Dataset Link List
Dataset FTP location
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Repository Record List
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