PXD000529-3

PXD000529 is an original dataset announced via ProteomeXchange.

Title	CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, instrument is Q Exactive, part 1 of 3
Description	The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-solution protein digestion by Lys-C and trypsin at 37 degrees Celsius, respectively. The tryptic peptides were cleaned by C18 Sep-Pak column (Waters UK Ltd, Manchester, UK). The mass spectrometer used in this dataset was Q-Exactive. The dataset was produced with two technical replicates and 24 fractions per repeat. The database searching procedure was achieved using Mascot v2.3. The database is Swiss-prot (release 2013 -6).
HostingRepository	PRIDE
AnnounceDate	2015-08-12
AnnouncementXML	Submission_2015-08-12_02:42:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000529
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zhang Chengpu Zhang Chengpu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	instrument model: Q-Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-18 02:57:57	ID requested
1	2013-12-13 10:19:25	announced
2	2015-08-12 02:36:18	announced	Updated project metadata.
⏵ 3	2015-08-12 02:42:36	announced	Updated project metadata.
4	2024-10-07 09:10:55	announced	2024-10-07: Updated project metadata.

Liu Y, Ying W, Ren Z, Gu W, Zhang Y, Yan G, Yang P, Liu Y, Yin X, Chang C, Jiang J, Fan F, Zhang C, Xu P, Wang Q, Wen B, Lin L, Wang T, Du C, Zhong J, Wang T, He QY, Qian X, Lou X, Zhang G, Zhong F, Chromosome-8-coded proteome of Chinese Chromosome Proteome Data set (CCPD) 2.0 with partial immunohistochemical verifications. J Proteome Res, 13(1):126-36(2014) [pubmed]

ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project

curator keyword: Biomedical

submitter keyword: CHPP, chromesome 1,8,20, HCC cell lines, Q-Exactive

Zhang Chengpu Zhang Chengpu
contact affiliation	Beijing proteome research center
contact email	zhangchengpu0203@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/12/PXD000529

PRIDE project URI

• PRIDE
  1. PXD000529
     1. Label: PRIDE project
     2. Name: CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, instrument is Q Exactive, part 1 of 3