PXD000341-1

PXD000341 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Site-specific quantitation of TCR-induced phosphorylation in Jurkat T cell lines
Description	The LC-MSMS data correspond to an investigation where we compared global dynamics of TCR signaling in Jurkat cell lines with or without LAT (Linker for activation of T cells). We used triple SILAC labelling. In each experiment two series of time points activation were generated with a common time point that we used to consolidate the two separate time series. The protein extracts in each time series were digested by trypsine and the peptide submitted to SCX fractionation and TiO2 enrichment of phosphopeptides. Each sample was injected 2-3 times. Raw data files were processed using an early version of MaxQuant software. In the first step, peak lists files (.msm files) for SILAC medium- and heavy-labeled peptide as well as unassigned labeling state were generated using the Quant.exe. These files were submitted to Mascot (version 2.3.01) search engine using Ensemble Human (GRCh37.59), in house generated, target decoy database.
HostingRepository	PRIDE
AnnounceDate	2013-11-15
AnnouncementXML	Submission_2013-11-15_03:22:17.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mogjib SALEK
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue; acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-15 00:44:13	ID requested
⏵ 1	2013-11-15 03:22:22	announced
⏵ 1	2013-11-15 03:28:19	announced
2	2014-07-25 01:22:38	announced	Updated project metadata.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Phosphoproteomics, T cell, Jurakts, LAT

submitter keyword: Phosphoproteomics, T cell, Jurakts, LAT

Contact List

Mogjib SALEK
contact affiliation	Sir William Dunn School of Pathology, Oxford (UK), and Proteomics Consulting, Toulouse, France.
contact email	mogjibs@gmail.com

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/11/PXD000341

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/11/PXD000341