PXD000305 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodelling in the Arabidopsis response to phosphate starvation |
Description | In order to identify new regulators of the phosphate (Pi) starvation signaling pathway in plants, we analyzed variation in the abundance of nuclear-enriched nuclear proteins isolated from Arabidopsis roots that depends on Pi supply. We used 2-D Fluorescence Difference Gel Electrophoresis and MALDI-TOF/TOF techniques for nuclear proteome separation, visualization and relative protein abundance quantification and identification. Pi-controlled proteins identified in our analysis included components of the chromatin remodeling, DNA replication, and mRNA splicing machineries. In addition, by combining Pi starvation conditions with proteasome inhibitor treatments, we characterized the role of the ubiquitin- (Ub)-proteasome system (UPS), a major mechanism for targeted protein degradation in eukaryotes, in the control of the stability of Pi-responsive proteins. Among Pi-responsive proteins, the histone chaperone NAP1;2 was selected for further characterization, and was shown to display differential nucleo-cytoplasmic accumulation in response to Pi deprivation. We also found that mutants affecting three members of the NAP1 family accumulate lower Pi levels and display reduced expression of Pi starvation-inducible genes, reflecting a regulatory role for these chromatin-remodelling proteins in Pi homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-22 |
AnnouncementXML | Submission_2020-01-22_04:55:16.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000305 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Vicente Rubio |
SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-06-27 05:05:31 | ID requested | |
⏵ 1 | 2020-01-22 04:55:17 | announced | |
Publication List
Iglesias J, Trigueros M, Rojas-Triana M, Fern, á, ndez M, Albar JP, Bustos R, Paz-Ares J, Rubio V, Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodeling in the Arabidopsis response to phosphate starvation. J Proteomics, 94():1-22(2013) [pubmed] |
Keyword List
submitter keyword: Phosphate starvation, Phosphate signaling, nuclear proteome, ubiquitin-proteasome system, NAP1;2, histone chaperone, DIGE, Arabidopsis |
Contact List
Vicente Rubio |
contact affiliation | Centro Nacional de Biotecnología (CNB-CSIC), Darwin, 3, 28049 Madrid, Spain |
contact email | vrubio@cnb.csic.es |
lab head | |
Vicente Rubio |
contact affiliation | Genetica Molecular de Plantas |
contact email | vrubio@cnb.csic.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000305
- Label: PRIDE project
- Name: Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodelling in the Arabidopsis response to phosphate starvation