PXD000114-1

PXD000114 is an original dataset announced via ProteomeXchange.

Title	Metaproteomics of Premature Infant Gut Colonization
Description	Microbial colonization of the human gastrointestinal tract plays an important role in establishing health and homeostasis. However, the time-dependent and related functional signatures of microbial and human proteins during early colonization of the gut have yet to be determined. Thus, we employed shotgun proteomics via nano-2D-LC-MS/MS to simultaneously monitor microbial and human proteins in fecal samples from a healthy preterm infant during early development. ). All MS/MS spectra were searched against a predicted protein database containing 25 microbial species along with the Human RefSeq2011 genome using the SEQUEST algorithm (Eng et al, 1994), and filtered with DTASelect version 1.9 (Tabb et al, 2002) at the peptide level with standard filters [SEQUEST Xcorrs of at least 1.8 (+1), 2.5 (+2) 3.5 (+3)] organizing identified peptides to their corresponding protein sequences. This study provides the first elucidation of coordinated human and microbial proteins in the infant gut during early development.
HostingRepository	PRIDE
AnnounceDate	2014-12-16
AnnouncementXML	Submission_2014-12-16_02:16:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacque Young
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2012-12-19 06:24:15	ID requested
⏵ 1	2014-12-16 02:16:22	announced
2	2024-10-22 04:16:53	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical, Metaproteomics

submitter keyword: colonization/ infant gut/ microbiome/ metaproteomics

Jacque Young
contact affiliation	Chemical Science Division
contact email	jcaprio@utk.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/12/PXD000114

PRIDE project URI

• PRIDE
  1. PXD000114
     1. Label: PRIDE project
     2. Name: Metaproteomics of Premature Infant Gut Colonization