PXD000026-1

PXD000026 is an original dataset announced via ProteomeXchange.

Title	Analysis of E2F2 interacting proteins
Description	In an attempt to identify proteins interacting with transcription factor E2F2, proteins extracted from HA-E2F2 overexpressing HEK293T cells were were subjected to immunoprecipitation (IP) with an anti-HA antibody or, as a negative control (NegCt), with an anti-T antibody. Immunoprecipitated proteins were eluted, digested with trypsin, and the resulting peptides were analyzed by LC-MS/MS. The whole procedure was repeated several times in an attempt to gauge reproducibility (nine replicates of the anti-HA IP and six replicates of the control IP).
HostingRepository	PRIDE
AnnounceDate	2013-01-21
AnnouncementXML	Submission_2013-01-21_05:49:03.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000026
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nerea Osinalde
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	quadrupole time-of-flight mass spectrometry

Revision	Datetime	Status	ChangeLog Entry
0	2012-09-11 09:23:41	ID requested
⏵ 1	2013-01-21 03:37:55	announced
⏵ 1	2014-07-28 00:21:04	announced
2	2013-01-21 05:49:03	announced	Add reference
3	2024-10-07 08:41:48	announced	2024-10-07: Updated project metadata.

Osinalde N, Olea M, Mitxelena J, Aloria K, Rodriguez JA, Fullaondo A, Arizmendi JM, Zubiaga AM, The nuclear protein ALY binds to and modulates the activity of transcription factor E2F2. Mol Cell Proteomics, 12(5):1087-98(2013) [pubmed]

submitter keyword: E2F2, immunoprecipitation, LC-MS/MS

Nerea Osinalde
contact affiliation	Biochemistry and Molecular Biology, University of the Basque Country
contact email	nerea.osinalde@ehu.es

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/01/PXD000026

• PRIDE
  1. 27228
     1. InstrumentRef: INSTRUMENT_1
     2. Label: IP32HA2
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Anti-HA immunoprecipitation - Biological replicate 2 - Biochemical replicate 2
     5. PublicationRef: PMID23297349
  2. 27238
     1. InstrumentRef: INSTRUMENT_1
     2. Label: IP33T3
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Anti-T immunoprecipitation - Biological replicate 2 - Biochemical replicate 3
     5. PublicationRef: PMID23297349
  3. 27229
     1. InstrumentRef: INSTRUMENT_1
     2. Label: IP32HA3
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Anti-HA immunoprecipitation - Biological replicate 2 - Biochemical replicate 3
     5. PublicationRef: PMID23297349
  4. 27224
     1. InstrumentRef: INSTRUMENT_1
     2. Label: IP30HA1
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Anti-HA immunoprecipitation - Biological replicate 1 - Biochemical replicate 1
     5. PublicationRef: PMID23297349
  5. 27236
     1. InstrumentRef: INSTRUMENT_1
     2. Label: IP33T1
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Anti-T immunoprecipitation - Biological replicate 2 - Biochemical replicate 1
     5. PublicationRef: PMID23297349
  6. 27232
     1. InstrumentRef: INSTRUMENT_1
     2. Label: IP32T3
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Anti-T immunoprecipitation - Biological replicate 1 - Biochemical replicate 3
     5. PublicationRef: PMID23297349
  7. 27225
     1. InstrumentRef: INSTRUMENT_1
     2. Label: IP30HA2
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Anti-HA immunoprecipitation - Biological replicate 1 - Biochemical replicate 2
     5. PublicationRef: PMID23297349
  8. 27235
     1. InstrumentRef: INSTRUMENT_1
     2. Label: IP33HA3
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Anti-HA immunoprecipitation - Biological replicate 3 - Biochemical replicate 3
     5. PublicationRef: PMID23297349
  9. 27226
     1. InstrumentRef: INSTRUMENT_1
     2. Label: IP30HA3
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Anti-HA immunoprecipitation - Biological replicate 1 - Biochemical replicate 3
     5. PublicationRef: PMID23297349
  10. 27227
      1. InstrumentRef: INSTRUMENT_1
      2. Label: IP32HA1
      3. ModificationList: iodoacetamide derivatized residue,
      4. Name: Anti-HA immunoprecipitation - Biological replicate 2 - Biochemical replicate 1
      5. PublicationRef: PMID23297349
  11. 27233
      1. InstrumentRef: INSTRUMENT_1
      2. Label: IP33HA1
      3. ModificationList: iodoacetamide derivatized residue,
      4. Name: Anti-HA immunoprecipitation - Biological replicate 3 - Biochemical replicate 1
      5. PublicationRef: PMID23297349
  12. 27230
      1. InstrumentRef: INSTRUMENT_1
      2. Label: IP32T1
      3. ModificationList: iodoacetamide derivatized residue,
      4. Name: Anti-T immunoprecipitation - Biological replicate 1 - Biochemical replicate 1
      5. PublicationRef: PMID23297349
  13. 27231
      1. InstrumentRef: INSTRUMENT_1
      2. Label: IP32T2
      3. ModificationList: iodoacetamide derivatized residue,
      4. Name: Anti-T immunoprecipitation - Biological replicate 1 - Biochemical replicate 2
      5. PublicationRef: PMID23297349
  14. 27237
      1. InstrumentRef: INSTRUMENT_1
      2. Label: IP33T2
      3. ModificationList: iodoacetamide derivatized residue,
      4. Name: Anti-T immunoprecipitation - Biological replicate 2 - Biochemical replicate 2
      5. PublicationRef: PMID23297349
  15. 27234
      1. InstrumentRef: INSTRUMENT_1
      2. Label: IP33HA2
      3. ModificationList: iodoacetamide derivatized residue,
      4. Name: Anti-HA immunoprecipitation - Biological replicate 3 - Biochemical replicate 2
      5. PublicationRef: PMID23297349